Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EG882_RS15420 Genome accession   NZ_CP033967
Coordinates   2963196..2963630 (-) Length   144 a.a.
NCBI ID   WP_015417516.1    Uniprot ID   -
Organism   Bacillus velezensis strain 1B-23     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2958196..2968630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG882_RS15385 (EG882_15385) - 2958209..2958562 (+) 354 WP_007410379.1 multidrug efflux SMR transporter -
  EG882_RS15390 (EG882_15390) - 2958576..2959028 (-) 453 WP_039251441.1 OsmC family protein -
  EG882_RS15395 (EG882_15395) - 2959088..2959795 (-) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  EG882_RS15400 (EG882_15400) - 2960051..2960284 (-) 234 WP_015239897.1 hypothetical protein -
  EG882_RS15405 (EG882_15405) - 2960463..2961791 (+) 1329 WP_124693027.1 S8 family peptidase -
  EG882_RS15410 (EG882_15410) - 2961985..2962347 (+) 363 WP_007410383.1 hypothetical protein -
  EG882_RS15415 (EG882_15415) - 2962381..2963136 (-) 756 WP_003154084.1 YoaK family protein -
  EG882_RS15420 (EG882_15420) nucA/comI 2963196..2963630 (-) 435 WP_015417516.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  EG882_RS15425 (EG882_15425) - 2963919..2965130 (+) 1212 WP_124693028.1 cytochrome P450 -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15514.44 Da        Isoelectric Point: 7.2418

>NTDB_id=327483 EG882_RS15420 WP_015417516.1 2963196..2963630(-) (nucA/comI) [Bacillus velezensis strain 1B-23]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=327483 EG882_RS15420 WP_015417516.1 2963196..2963630(-) (nucA/comI) [Bacillus velezensis strain 1B-23]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment