Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EG882_RS01585 Genome accession   NZ_CP033967
Coordinates   300804..301241 (+) Length   145 a.a.
NCBI ID   WP_069007410.1    Uniprot ID   -
Organism   Bacillus velezensis strain 1B-23     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 295804..306241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG882_RS01570 (EG882_01570) - 298664..299026 (-) 363 WP_012116770.1 helix-turn-helix domain-containing protein -
  EG882_RS01575 (EG882_01575) hxlA 299258..299893 (+) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  EG882_RS01580 (EG882_01580) hxlB 299890..300447 (+) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  EG882_RS01585 (EG882_01585) nucA/comI 300804..301241 (+) 438 WP_069007410.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  EG882_RS01590 (EG882_01590) nin/comJ 301262..301660 (+) 399 WP_014416873.1 competence protein ComJ Regulator
  EG882_RS01595 (EG882_01595) - 301701..303137 (-) 1437 WP_025284208.1 family 1 glycosylhydrolase -
  EG882_RS01600 (EG882_01600) - 303241..303564 (-) 324 WP_007409357.1 YckD family protein -
  EG882_RS01605 (EG882_01605) - 303636..304070 (-) 435 WP_007409358.1 RDD family protein -
  EG882_RS01610 (EG882_01610) - 304085..305221 (-) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16041.39 Da        Isoelectric Point: 9.3990

>NTDB_id=327436 EG882_RS01585 WP_069007410.1 300804..301241(+) (nucA/comI) [Bacillus velezensis strain 1B-23]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKKGHSGICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=327436 EG882_RS01585 WP_069007410.1 300804..301241(+) (nucA/comI) [Bacillus velezensis strain 1B-23]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAAAAGGACATTCCGGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634

Multiple sequence alignment