Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   TGR7_RS03885 Genome accession   NC_011901
Coordinates   844532..845398 (-) Length   288 a.a.
NCBI ID   WP_012637365.1    Uniprot ID   B8GMP5
Organism   Thioalkalivibrio sulfidiphilus HL-EbGr7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 839532..850398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TGR7_RS03860 (Tgr7_0780) argJ 840019..841230 (+) 1212 WP_012637360.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  TGR7_RS03865 (Tgr7_0781) - 841365..842318 (+) 954 WP_012637361.1 Nudix family hydrolase -
  TGR7_RS03870 (Tgr7_0782) - 842315..842788 (+) 474 WP_012637362.1 hypothetical protein -
  TGR7_RS03875 (Tgr7_0783) zapD 842917..843759 (-) 843 WP_049764610.1 cell division protein ZapD -
  TGR7_RS03880 (Tgr7_0784) coaE 843796..844401 (-) 606 WP_012637364.1 dephospho-CoA kinase -
  TGR7_RS03885 (Tgr7_0785) pilD 844532..845398 (-) 867 WP_012637365.1 A24 family peptidase Machinery gene
  TGR7_RS03890 (Tgr7_0786) pilC 845507..846718 (-) 1212 WP_012637366.1 type II secretion system F family protein Machinery gene
  TGR7_RS03895 (Tgr7_0787) pilB 846723..848438 (-) 1716 WP_041440910.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31394.27 Da        Isoelectric Point: 7.3222

>NTDB_id=32735 TGR7_RS03885 WP_012637365.1 844532..845398(-) (pilD) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MIELLQTSTAALLTVTALFSLLVGSFLNVVIHRLPVMMERQWRREATEFLYAEGQTLAPAEQGPAYNLWSPRSACPQCGH
KITALENIPILSYLLLRGRCSECGTSIPLRYPLVEAATALLSVVVVWHFGFTWQSGAALLLTWALIALAVIDLRTTLLPD
SITLPFLWIGLLLNLGGLFTDATSSLIGAVAGYLSLWLVYHGFKLLTGKEGMGFGDFKLFALIGAWLGWQLLPLVILLSS
LVGAVVGIALILFRGRDRAHPMPFGPYLAAAGWIALLWGNDIMALYLG

Nucleotide


Download         Length: 867 bp        

>NTDB_id=32735 TGR7_RS03885 WP_012637365.1 844532..845398(-) (pilD) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGATTGAACTCCTGCAAACCAGCACGGCGGCACTGCTGACCGTCACCGCACTGTTCAGCCTGCTGGTGGGCAGCTTTCT
CAATGTGGTGATTCATCGTCTGCCGGTGATGATGGAGCGGCAGTGGCGGCGTGAAGCCACGGAATTCCTGTACGCCGAGG
GCCAGACGCTTGCGCCAGCCGAGCAGGGGCCCGCCTACAACCTCTGGAGCCCCCGTTCCGCCTGTCCCCAGTGCGGTCAC
AAGATCACCGCGCTGGAGAACATCCCGATCCTCAGCTACCTGCTCCTGCGCGGCCGTTGCAGCGAGTGCGGCACCTCGAT
TCCGCTGCGCTACCCGCTGGTGGAGGCGGCCACGGCTCTGTTGTCCGTGGTAGTGGTCTGGCACTTCGGCTTCACCTGGC
AGTCGGGGGCCGCGCTGCTGCTCACCTGGGCACTGATCGCGCTGGCGGTCATCGACCTGCGCACCACCCTGCTCCCGGAC
AGCATCACCCTGCCCTTCCTCTGGATCGGCCTGTTACTCAACCTGGGCGGCCTGTTCACCGACGCCACCAGCAGCCTGAT
CGGCGCCGTGGCCGGTTACCTGAGCCTCTGGCTGGTGTATCACGGCTTCAAGCTGCTCACCGGCAAGGAAGGCATGGGCT
TCGGCGACTTCAAGCTCTTCGCCCTCATCGGCGCCTGGCTCGGCTGGCAGCTGCTGCCCCTGGTGATCCTGCTCTCATCC
CTGGTAGGCGCCGTGGTGGGCATCGCGCTGATCCTGTTCCGCGGCCGCGACCGCGCCCATCCCATGCCCTTCGGCCCCTA
CCTGGCAGCGGCCGGCTGGATCGCGCTGCTGTGGGGCAACGACATCATGGCGCTGTACCTCGGGTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-129)

Type IV leader peptidase family (Peptidase_A24)

(140-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8GMP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.986

96.181

0.5
  pilD Vibrio campbellii strain DS40M4

49.129

99.653

0.49
  pilD Acinetobacter baumannii D1279779

49.11

97.569

0.479
  pilD Acinetobacter nosocomialis M2

49.11

97.569

0.479
  pilD Neisseria gonorrhoeae MS11

50.775

89.583

0.455

Multiple sequence alignment