Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EG347_RS10790 Genome accession   NZ_CP033918
Coordinates   2445278..2446627 (+) Length   449 a.a.
NCBI ID   WP_123943155.1    Uniprot ID   A0A3G6PCL4
Organism   Chryseobacterium sp. G0186     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2440278..2451627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG347_RS10770 (EG347_10880) - 2440702..2441457 (+) 756 WP_123943147.1 SDR family NAD(P)-dependent oxidoreductase -
  EG347_RS10775 (EG347_10885) - 2441439..2442740 (+) 1302 WP_123943149.1 phosphatase PAP2 family protein -
  EG347_RS10780 (EG347_10890) - 2442744..2444444 (+) 1701 WP_123943151.1 bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase -
  EG347_RS10785 (EG347_10895) - 2444569..2445135 (-) 567 WP_123943153.1 YceI family protein -
  EG347_RS10790 (EG347_10900) radA 2445278..2446627 (+) 1350 WP_123943155.1 DNA repair protein RadA Machinery gene
  EG347_RS10795 (EG347_10905) - 2446631..2447299 (+) 669 WP_228452054.1 class I SAM-dependent DNA methyltransferase -
  EG347_RS10800 (EG347_10910) - 2447296..2448003 (+) 708 WP_123943159.1 VanW family protein -
  EG347_RS10805 (EG347_10915) - 2448000..2448593 (+) 594 WP_123943161.1 ACP phosphodiesterase -
  EG347_RS10810 (EG347_10920) - 2448590..2449105 (-) 516 WP_123943163.1 DUF6702 family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 49898.48 Da        Isoelectric Point: 6.5083

>NTDB_id=327089 EG347_RS10790 WP_123943155.1 2445278..2446627(+) (radA) [Chryseobacterium sp. G0186]
MAKLKTAYFCQNCGTQYSQWMGQCKNCGEWNTLVEEVVEKTSHKAVPFSKTKQHVINIIEVETSEEPRIKTPSEELNRVL
GGGIVLGSVTLIGGEPGIGKSTLLLQLALKMKKKIFYVSGEESASQIKMRADRLTDIQNPNCFLFTETSLEKILHEAKKL
EPDFMIIDSIQTLQSQLIESSPGTVSQIRECSNEIIKYAKENSIPVFLVGHITKDGQIAGPKVLEHMVDVVLNFDGDRNH
LFRLLRANKNRFGSTSEIGIYEMVSQGLKEIKNPSEILITKKFEELSGNSVAVTLEGNRPMLLEIQALVSTAVYGTPQRS
STGFDSKRLNMLLAVLEKRAGFQLGAKDVFLNITGGIKTDDPALDLAVIASVLSSNEDIAISEHYCFAGEIGLSGEIRPV
AQAEQRITEAEKLGYEKIFVSNLNKIPKRKFGIKIEEVSKIEDFHERLF

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=327089 EG347_RS10790 WP_123943155.1 2445278..2446627(+) (radA) [Chryseobacterium sp. G0186]
ATGGCAAAACTGAAAACGGCATATTTCTGTCAAAACTGCGGAACACAATATTCTCAATGGATGGGACAATGCAAAAATTG
TGGAGAATGGAATACCTTAGTGGAAGAAGTTGTGGAAAAGACTTCTCACAAGGCTGTACCGTTCTCAAAAACCAAGCAAC
ACGTCATCAATATCATTGAGGTGGAAACCAGTGAAGAACCAAGAATAAAAACGCCGTCCGAAGAACTGAACCGCGTGTTG
GGAGGGGGAATTGTACTAGGTTCCGTTACCCTGATCGGCGGTGAACCGGGAATCGGGAAATCTACTCTTCTGTTGCAGCT
TGCCTTAAAAATGAAGAAAAAAATCTTCTATGTTTCCGGGGAAGAAAGTGCCTCTCAGATTAAAATGAGAGCCGACAGAC
TAACGGATATTCAAAATCCAAACTGTTTTCTTTTTACTGAAACCTCACTGGAAAAAATTCTTCACGAAGCCAAAAAACTG
GAGCCGGATTTTATGATCATCGATTCCATCCAGACGCTTCAGTCCCAGCTTATCGAAAGCTCACCGGGAACAGTTTCCCA
AATCCGGGAATGCTCCAATGAGATTATTAAATATGCCAAGGAAAATAGTATTCCTGTATTTTTGGTAGGTCACATCACCA
AAGATGGTCAGATTGCCGGGCCAAAGGTTTTGGAACACATGGTAGATGTGGTTTTAAACTTTGACGGAGACCGAAATCAT
CTTTTCAGACTATTGAGGGCCAATAAAAACCGTTTTGGTTCTACTTCTGAAATTGGAATTTATGAAATGGTTTCCCAGGG
ATTAAAAGAAATAAAAAATCCGTCTGAAATTCTTATCACAAAGAAATTTGAGGAACTTTCCGGAAACTCAGTTGCCGTAA
CTTTGGAGGGAAACAGACCTATGCTTCTGGAAATCCAGGCATTGGTAAGTACCGCGGTTTATGGTACACCACAGAGAAGC
TCTACAGGATTTGATTCAAAAAGACTGAATATGCTTTTGGCGGTGCTTGAAAAACGAGCTGGCTTCCAACTGGGTGCCAA
GGACGTCTTTTTAAATATTACAGGAGGAATAAAAACAGATGATCCCGCCCTGGATTTGGCAGTCATTGCATCTGTTCTCT
CATCTAATGAAGATATCGCTATTTCTGAACATTACTGCTTTGCCGGAGAGATTGGATTAAGTGGAGAAATTCGCCCGGTT
GCTCAAGCTGAACAGAGAATTACTGAAGCTGAAAAACTTGGGTATGAAAAGATATTCGTTTCCAATCTTAATAAAATTCC
GAAGAGAAAATTCGGAATCAAAATTGAAGAGGTGAGTAAAATTGAGGATTTCCACGAAAGGCTTTTCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(338-423)

AAA domain (AAA_25)

(65-216)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G6PCL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.894

100

0.492
  radA Streptococcus pneumoniae Rx1

48.246

100

0.49
  radA Streptococcus pneumoniae D39

48.246

100

0.49
  radA Streptococcus pneumoniae R6

48.246

100

0.49
  radA Streptococcus pneumoniae TIGR4

48.246

100

0.49
  radA Streptococcus mitis NCTC 12261

48.246

100

0.49
  radA Streptococcus mitis SK321

50.118

94.209

0.472

Multiple sequence alignment