Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EHF40_RS06355 Genome accession   NZ_CP033907
Coordinates   1213532..1215337 (-) Length   601 a.a.
NCBI ID   WP_002995768.1    Uniprot ID   A0A660A6L4
Organism   Streptococcus pyogenes strain Duke-Large     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1189882..1223151 1213532..1215337 within 0


Gene organization within MGE regions


Location: 1189882..1223151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHF40_RS06265 (EHF40_06265) - 1189883..1191340 (-) 1458 Protein_1157 IS1182 family transposase -
  EHF40_RS06270 (EHF40_06270) - 1191507..1192781 (-) 1275 WP_011528746.1 polysaccharide deacetylase family protein -
  EHF40_RS06275 (EHF40_06275) - 1192875..1194302 (-) 1428 WP_002989248.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  EHF40_RS06280 (EHF40_06280) ptsP 1194487..1196220 (-) 1734 WP_011528747.1 phosphoenolpyruvate--protein phosphotransferase -
  EHF40_RS06285 (EHF40_06285) - 1196225..1196488 (-) 264 WP_002984031.1 phosphocarrier protein HPr -
  EHF40_RS06290 (EHF40_06290) - 1196881..1197099 (+) 219 WP_042769861.1 redoxin NrdH -
  EHF40_RS06295 (EHF40_06295) nrdE 1197119..1199278 (+) 2160 WP_011528750.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  EHF40_RS06300 (EHF40_06300) nrdF 1199869..1200828 (+) 960 WP_011017933.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  EHF40_RS06305 (EHF40_06305) - 1200825..1202300 (-) 1476 Protein_1165 IS1182 family transposase -
  EHF40_RS06310 (EHF40_06310) - 1202448..1203674 (+) 1227 WP_021299359.1 chloride channel protein -
  EHF40_RS06315 (EHF40_06315) - 1203770..1204920 (+) 1151 Protein_1167 IS3 family transposase -
  EHF40_RS06320 (EHF40_06320) - 1205017..1205712 (-) 696 WP_011528757.1 type II CAAX endopeptidase family protein -
  EHF40_RS06325 (EHF40_06325) - 1205731..1206492 (-) 762 WP_011528758.1 DUF3169 family protein -
  EHF40_RS06330 (EHF40_06330) - 1206489..1206704 (-) 216 WP_002989220.1 helix-turn-helix transcriptional regulator -
  EHF40_RS06335 (EHF40_06335) alaS 1207064..1209682 (-) 2619 WP_011528759.1 alanine--tRNA ligase -
  EHF40_RS06340 (EHF40_06340) prsA 1210069..1211124 (-) 1056 WP_002984003.1 peptidylprolyl isomerase PrsA -
  EHF40_RS06345 (EHF40_06345) - 1211187..1211894 (-) 708 WP_011528760.1 O-methyltransferase -
  EHF40_RS06350 (EHF40_06350) - 1211960..1213156 (-) 1197 WP_002995765.1 OFA family MFS transporter -
  EHF40_RS06355 (EHF40_06355) pepF 1213532..1215337 (-) 1806 WP_002995768.1 oligoendopeptidase F Regulator
  EHF40_RS06360 (EHF40_06360) - 1215350..1216312 (-) 963 WP_002983995.1 competence protein CoiA -
  EHF40_RS06365 (EHF40_06365) - 1216607..1217323 (-) 717 WP_011528761.1 pseudouridine synthase -
  EHF40_RS06370 (EHF40_06370) nagB 1217442..1218146 (-) 705 WP_021299319.1 glucosamine-6-phosphate deaminase -
  EHF40_RS06375 (EHF40_06375) queA 1218348..1219376 (+) 1029 WP_121158093.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  EHF40_RS06380 (EHF40_06380) - 1219383..1220603 (+) 1221 WP_011528764.1 DUF3114 domain-containing protein -
  EHF40_RS06385 (EHF40_06385) - 1220717..1221552 (-) 836 Protein_1181 serine hydrolase -
  EHF40_RS06395 (EHF40_06395) - 1221608..1221754 (-) 147 WP_011528767.1 hypothetical protein -
  EHF40_RS06400 (EHF40_06400) sodA 1221921..1222526 (-) 606 WP_002983977.1 superoxide dismutase SodA -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69394.45 Da        Isoelectric Point: 4.8408

>NTDB_id=326921 EHF40_RS06355 WP_002995768.1 1213532..1215337(-) (pepF) [Streptococcus pyogenes strain Duke-Large]
MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFAGHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKND
QDTTVAKYQEYQAKASGLYAKFSEVFSFYDPEVMMLHQEDYQAFLTETPELKVYNHFFDKLFQAREHVLSQAEEELLAGA
QEIFNGAEETFSILDNADIVFPIVRNDKGENVELTHGNFISLMESKDRSVRQAAYEAMYSTYEQFQHTYAKTLQTNVKVQ
NYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNKHLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALE
KAEKVLAVFGKDYADRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSLHSTFTRET
QPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFRGTVFRQTQFAEFEHAIHQADQKGEVLTSEY
LNQLYADLNEKYYGLSKKDNHFIQYEWARIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPL
EVIAKAGVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=326921 EHF40_RS06355 WP_002995768.1 1213532..1215337(-) (pepF) [Streptococcus pyogenes strain Duke-Large]
ATGACTGATAATCGCAGCCATTTAGAAGAAAAATACACTTGGGATTTGAGCACTATTTTTGCAACAGATAAGGATTGGGA
AGCTGAAGTCAGTGACTTAGCTACTGAAGTTGAGGCATCTAAAGGTTTTGCTGGTCATTTGCTGGATTCTAGTGCTAATT
TGTTAAAGGTTACAAAAACATATTTAGAATTGGCTCGTCGTGTGGAGAAAGTCTATGTTTATGCTCACATGAAAAATGAC
CAAGACACTACTGTTGCCAAGTATCAAGAATACCAAGCTAAGGCTTCAGGCTTGTATGCAAAGTTTAGCGAAGTCTTTTC
ATTCTATGATCCAGAAGTGATGATGCTTCATCAAGAAGACTATCAGGCTTTCCTTACTGAAACCCCTGAGTTGAAAGTGT
ACAACCATTTCTTTGACAAATTATTCCAAGCCCGCGAACATGTTCTTAGTCAAGCAGAAGAGGAATTATTAGCAGGAGCT
CAAGAAATTTTCAATGGTGCGGAAGAAACCTTTAGTATTCTTGATAACGCTGACATTGTTTTCCCGATAGTCAGAAATGA
TAAAGGTGAAAACGTTGAATTAACCCATGGTAACTTCATTAGCCTAATGGAATCTAAGGACCGTAGCGTTCGCCAAGCTG
CTTATGAAGCCATGTATAGTACTTATGAACAGTTCCAACATACTTACGCTAAAACCTTGCAAACCAATGTGAAAGTGCAA
AACTATAAGGCGCGTGTTCATAAATACGATTCAGCACGTCAAGCTGCCATGGCTGCTAACTTTATTCCAGAAGCTGTTTA
CGACACTTTATTAGAAACGGTTAACAAACATTTACCACTCTTACACCGTTATTTGAAATTGCGTCAAGAGGTTCTTGGTT
TAGACGATTTGAAGATGTATGATGTTTACACGCCATTATCTGAAACAGATTTAGCCATTGGTTATGATGAGGCTTTGGAA
AAGGCTGAGAAGGTTTTGGCTGTTTTTGGTAAGGATTACGCAGATCGTGTACATCGTGCTTTTACCGAACGTTGGATTGA
TGTTCACGTCAATAAAGGGAAGCGCTCTGGGGCTTATTCAGGTGGTTCCTATGATACCAATGCCTTCATGCTGTTGAACT
GGCAAGATACACTTGATAATCTTTACACACTTGTCCACGAAACAGGCCACAGTTTGCATTCTACTTTCACTCGTGAAACA
CAGCCTTATGTGTATGGGGATTATAGTATCTTCTTGGCAGAAATTGCATCCACAACGAATGAAAATATTATGACAGAAGC
TCTCTTAAATGAGGTTCAAGATGAGAAAGAACGCTTTGCCATTTTAAACCATTACCTCGATGGTTTCCGTGGAACAGTTT
TCCGTCAAACCCAATTTGCTGAGTTTGAACATGCCATTCATCAAGCTGATCAAAAAGGAGAAGTGTTAACAAGTGAATAC
CTTAACCAGCTATACGCTGATTTAAATGAAAAATATTATGGTTTAAGTAAAAAAGATAATCATTTTATTCAGTATGAATG
GGCACGTATTCCTCATTTCTATTACAATTATTATGTTTACCAATATGCAACAGGGTTTGCAGCTGCAAGCTATTTAGCGG
ATAAGATTGTGCATGGCACACAAGATGATATCGATCATTACTTGGCCTACCTTAAGTCAGGAAATTCTGATTACCCACTT
GAAGTGATTGCTAAAGCAGGCGTTGATATGGCAAAAGGTGATTATTTGGAAGCCGCCTTTAAAGTCTTTGACGAACGTCT
AACAGAACTTGAAGTACTCGTGTCCAAAGGCATACACAAAAGCTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(112-181)

Peptidase family M3 (Peptidase_M3)

(202-581)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A660A6L4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

70.715

100

0.707

Multiple sequence alignment