Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EGU21_RS05355 Genome accession   NZ_CP033885
Coordinates   1168774..1170150 (+) Length   458 a.a.
NCBI ID   WP_024525645.1    Uniprot ID   A0A1X0XL19
Organism   Levilactobacillus brevis strain LMT1-73     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1163774..1175150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGU21_RS05330 - 1163798..1165030 (+) 1233 WP_069360034.1 serine hydrolase domain-containing protein -
  EGU21_RS05335 - 1165165..1166487 (-) 1323 WP_369707378.1 C1 family peptidase -
  EGU21_RS12390 - 1166645..1166815 (+) 171 WP_164475118.1 hypothetical protein -
  EGU21_RS05340 rpiA 1166926..1167612 (-) 687 WP_011667345.1 ribose-5-phosphate isomerase RpiA -
  EGU21_RS05345 - 1167620..1167961 (-) 342 WP_024525647.1 hypothetical protein -
  EGU21_RS05350 - 1168140..1168679 (+) 540 WP_024525646.1 dUTP diphosphatase -
  EGU21_RS05355 radA 1168774..1170150 (+) 1377 WP_024525645.1 DNA repair protein RadA Machinery gene
  EGU21_RS05360 - 1170186..1171370 (+) 1185 WP_021741476.1 PIN/TRAM domain-containing protein -
  EGU21_RS05365 gltX 1171634..1173124 (+) 1491 WP_011667350.1 glutamate--tRNA ligase -
  EGU21_RS05370 cysS 1173279..1174691 (+) 1413 WP_123830919.1 cysteine--tRNA ligase -
  EGU21_RS05375 - 1174691..1175098 (+) 408 WP_011667352.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49788.09 Da        Isoelectric Point: 7.0881

>NTDB_id=326764 EGU21_RS05355 WP_024525645.1 1168774..1170150(+) (radA) [Levilactobacillus brevis strain LMT1-73]
MAKPRTTYVCQNCDYSSPRYLGRCPNCGEWNTMVEEVVTPAAAKPQSTRTTASGERSHPQLMSEIKHSTESRTKTQMEEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSQTGGKVLYVSGEESASQIKMRADRLVVNSDHLYLYPETDMASIR
ANIEQMKPDYVVIDSVQTMQAPGIESAIGSVSQIRAVTGELMQIAKTNGITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRAVKNRFGSTNELGIFEMREGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALITPSVF
GNAQRTSSGLDRNRVSLLMAVLEKRANLMLQNQDAFLKAAGGVKLDEPAIDLAIALSIASSYRDTATQPTDCFVGEVGLT
GEIRRVNRIEQRVAEAKKLGFKRIFIPKNNLQGWTPDPGIDVVGVSTLRQTLKLALDV

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=326764 EGU21_RS05355 WP_024525645.1 1168774..1170150(+) (radA) [Levilactobacillus brevis strain LMT1-73]
GTGGCGAAACCAAGAACAACGTATGTGTGTCAAAATTGTGATTATAGCTCACCCCGTTATTTGGGGCGTTGTCCCAATTG
TGGTGAGTGGAACACGATGGTGGAAGAAGTCGTCACACCGGCTGCCGCCAAGCCACAATCAACCCGGACCACTGCTTCCG
GAGAACGGTCACACCCACAACTCATGAGTGAGATTAAACACTCAACGGAAAGCCGAACGAAGACGCAAATGGAAGAACTG
AACCGAGTGTTGGGTGGGGGAATTGTACCGGGATCTTTGATCCTAATCGGTGGTGATCCTGGAATTGGAAAATCAACCCT
CTTGCTTCAAGTATCGGGGCAACTCAGCCAAACAGGCGGTAAAGTGTTGTATGTTTCTGGAGAAGAAAGTGCGTCACAGA
TTAAAATGCGGGCGGATCGGTTAGTCGTCAATAGCGACCACTTGTATCTTTATCCAGAAACTGATATGGCCAGTATTCGC
GCGAACATTGAACAGATGAAACCTGATTACGTGGTCATTGATTCCGTTCAGACCATGCAGGCGCCGGGAATCGAGTCGGC
GATTGGTTCGGTCTCACAAATTCGTGCGGTCACTGGTGAGCTGATGCAGATTGCCAAAACCAATGGCATCACTATTTTTG
TGGTGGGGCATGTGACGAAGGGCGGCGCAATTGCCGGTCCTAAGATTCTGGAACACATGGTAGATACGGTTCTTTACTTT
GAAGGTGACCTCCATCATACGTACCGAATTCTCCGAGCGGTCAAGAACCGATTCGGGTCAACGAATGAACTGGGTATCTT
TGAAATGCGTGAAGGTGGCTTGTATGAAGTTGCTAATCCGTCAGAAATTTTCTTGGAGGAACGATTGAAGGACGCAACCG
GTTCGGCCATTGTGGTATCAATGGAAGGTACGCGGCCAATTTTGGTAGAAGTTCAGGCGTTAATTACACCATCGGTCTTT
GGTAATGCGCAACGCACATCCAGCGGCCTGGATCGTAATCGGGTGTCGTTGTTGATGGCTGTGCTAGAAAAACGGGCCAA
TCTAATGCTCCAAAATCAAGATGCATTTTTAAAGGCAGCAGGTGGCGTTAAGCTGGATGAACCGGCGATTGATTTGGCAA
TTGCACTGTCGATTGCATCGAGTTATCGCGATACGGCCACGCAGCCAACGGATTGTTTTGTCGGTGAAGTTGGCCTGACT
GGTGAAATCCGTCGGGTTAATCGAATTGAACAACGGGTTGCGGAAGCGAAAAAACTGGGCTTTAAGCGGATCTTCATTCC
CAAGAATAACCTTCAAGGGTGGACGCCCGATCCCGGAATTGATGTGGTCGGCGTGTCAACGTTACGACAAACGTTAAAAC
TGGCTTTGGACGTGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X0XL19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.667

98.908

0.659
  radA Streptococcus mitis NCTC 12261

66.446

98.908

0.657
  radA Streptococcus pneumoniae Rx1

66.446

98.908

0.657
  radA Streptococcus pneumoniae D39

66.446

98.908

0.657
  radA Streptococcus pneumoniae R6

66.446

98.908

0.657
  radA Streptococcus pneumoniae TIGR4

66.446

98.908

0.657
  radA Bacillus subtilis subsp. subtilis str. 168

64.035

99.563

0.638

Multiple sequence alignment