Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EGM96_RS19140 Genome accession   NZ_CP033876
Coordinates   3962394..3963194 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain WU_164     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3957394..3968194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM96_RS19120 (EGM96_19235) - 3958459..3959157 (-) 699 WP_000058788.1 YfgM family protein -
  EGM96_RS19125 (EGM96_19240) hisS 3959186..3960478 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  EGM96_RS19130 (EGM96_19245) ispG 3960475..3961590 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EGM96_RS19135 (EGM96_19250) - 3961612..3962403 (-) 792 WP_031959662.1 helix-turn-helix domain-containing protein -
  EGM96_RS19140 (EGM96_19255) pilF 3962394..3963194 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EGM96_RS19145 (EGM96_19260) rlmN 3963210..3964442 (-) 1233 WP_031959661.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EGM96_RS19150 (EGM96_19265) ndk 3964565..3964996 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  EGM96_RS19155 (EGM96_19270) iscX 3965110..3965310 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  EGM96_RS19160 (EGM96_19275) - 3965410..3965961 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  EGM96_RS19165 (EGM96_19280) - 3965986..3967284 (-) 1299 WP_109067365.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=326735 EGM96_RS19140 WP_004738086.1 3962394..3963194(-) (pilF) [Acinetobacter baumannii strain WU_164]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=326735 EGM96_RS19140 WP_004738086.1 3962394..3963194(-) (pilF) [Acinetobacter baumannii strain WU_164]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAGCAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment