Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EGM95_RS18030 Genome accession   NZ_CP033869
Coordinates   3655100..3655900 (-) Length   266 a.a.
NCBI ID   WP_002027454.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain MRSN15313     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3650100..3660900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM95_RS18010 (EGM95_18010) - 3651165..3651863 (-) 699 WP_000058788.1 YfgM family protein -
  EGM95_RS18015 (EGM95_18015) hisS 3651892..3653184 (-) 1293 WP_000095264.1 histidine--tRNA ligase -
  EGM95_RS18020 (EGM95_18020) ispG 3653181..3654296 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EGM95_RS18025 (EGM95_18025) - 3654318..3655109 (-) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  EGM95_RS18030 (EGM95_18030) pilF 3655100..3655900 (-) 801 WP_002027454.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EGM95_RS18035 (EGM95_18035) rlmN 3655916..3657148 (-) 1233 WP_000093083.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EGM95_RS18040 (EGM95_18040) ndk 3657271..3657702 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  EGM95_RS18045 (EGM95_18045) iscX 3657816..3658016 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  EGM95_RS18050 (EGM95_18050) - 3658116..3658667 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  EGM95_RS18055 (EGM95_18055) - 3658692..3659990 (-) 1299 WP_086242039.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30124.47 Da        Isoelectric Point: 9.2307

>NTDB_id=326672 EGM95_RS18030 WP_002027454.1 3655100..3655900(-) (pilF) [Acinetobacter baumannii strain MRSN15313]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=326672 EGM95_RS18030 WP_002027454.1 3655100..3655900(-) (pilF) [Acinetobacter baumannii strain MRSN15313]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCTAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment