Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FNE86_RS18085 Genome accession   NZ_CP041422
Coordinates   3696928..3697368 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain STEC409     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3691928..3702368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNE86_RS18065 (FNE86_18305) aroP 3692926..3694299 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  FNE86_RS18070 (FNE86_18310) ampE 3694342..3695196 (-) 855 Protein_3574 beta-lactamase regulator AmpE -
  FNE86_RS18075 (FNE86_18315) ampD 3695193..3695744 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FNE86_RS18080 (FNE86_18320) nadC 3695832..3696725 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FNE86_RS18085 (FNE86_18325) pilA 3696928..3697368 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FNE86_RS18090 (FNE86_18330) pilB 3697378..3698763 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  FNE86_RS18095 (FNE86_18335) hofC 3698753..3699955 (+) 1203 WP_032083626.1 protein transport protein HofC -
  FNE86_RS18100 (FNE86_18340) guaC 3699990..3701033 (-) 1044 WP_001217337.1 GMP reductase -
  FNE86_RS23925 - 3701189..3701233 (-) 45 WP_120795372.1 protein YacM -
  FNE86_RS18105 (FNE86_18345) coaE 3701258..3701878 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=326134 FNE86_RS18085 WP_000360904.1 3696928..3697368(+) (pilA) [Escherichia coli strain STEC409]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=326134 FNE86_RS18085 WP_000360904.1 3696928..3697368(+) (pilA) [Escherichia coli strain STEC409]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment