Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EGX82_RS05220 Genome accession   NZ_CP033822
Coordinates   1004067..1005431 (-) Length   454 a.a.
NCBI ID   WP_001085193.1    Uniprot ID   A0AAW6XZY4
Organism   Streptococcus agalactiae strain FDAARGOS_512     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 999067..1010431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGX82_RS05200 (EGX82_05200) - 999290..1000258 (+) 969 WP_000668261.1 substrate-binding domain-containing protein -
  EGX82_RS05205 (EGX82_05205) gltX 1000298..1001752 (-) 1455 WP_001284505.1 glutamate--tRNA ligase -
  EGX82_RS05210 (EGX82_05210) - 1001961..1003280 (-) 1320 WP_000759708.1 FAD-containing oxidoreductase -
  EGX82_RS05215 (EGX82_05215) - 1003434..1003931 (-) 498 WP_000214746.1 carbonic anhydrase -
  EGX82_RS05220 (EGX82_05220) radA 1004067..1005431 (-) 1365 WP_001085193.1 DNA repair protein RadA Machinery gene
  EGX82_RS05225 (EGX82_05225) - 1005593..1006039 (-) 447 WP_000049182.1 dUTP diphosphatase -
  EGX82_RS05235 (EGX82_05235) - 1006234..1007160 (-) 927 WP_000723853.1 alpha/beta hydrolase -
  EGX82_RS05240 (EGX82_05240) - 1007269..1008873 (-) 1605 WP_000170432.1 CTP synthase -
  EGX82_RS05245 (EGX82_05245) rpoE 1009146..1009721 (-) 576 WP_000418426.1 DNA-directed RNA polymerase subunit delta -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49562.88 Da        Isoelectric Point: 6.8566

>NTDB_id=326127 EGX82_RS05220 WP_001085193.1 1004067..1005431(-) (radA) [Streptococcus agalactiae strain FDAARGOS_512]
MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKNARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPKESFIGEIGLTGEIR
RVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKAVFST

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=326127 EGX82_RS05220 WP_001085193.1 1004067..1005431(-) (radA) [Streptococcus agalactiae strain FDAARGOS_512]
GTGGCTAAGAAAAAATCAGTCTTCACCTGTCAAGAGTGTGGTTACCAATCACCTAAGTATTTGGGACGTTGTCCAAATTG
CTCAGCCTGGTCTTCATTTGTAGAAGAGGTTGAAGTTCAAGAAGTTAAAAATGCACGAGTTAGTTTGAACGGTGAAAAGT
CCCGACCGACGAAGTTAAAAGATGTTTCCTCAATTAACTATTCTAGAACTAAGACTGATATGGATGAATTTAACCGTGTT
CTAGGAGGCGGCGTTGTACCAGGAAGTTTGGTTCTGATTGGAGGAGACCCTGGGATAGGTAAATCTACTTTATTACTACA
AGTATCAACTCAGCTAGCTAATAAAGGTACAGTATTATATGTCTCAGGTGAGGAATCTGCAGAACAGATTAAGTTACGAA
GTGAGCGTCTAGGCGATATCGATAATGAATTTTACCTTTATGCCGAGACTAATATGCAAAGTATTCGGTCGGAGATTGAG
AAGATTAAACCAGATTTTCTTATTATTGATTCTATACAAACTATTATGAGTCCCGAAGTATCAAGTGTCCAAGGCTCTGT
TAGCCAAGTTCGCGAAGTGACCGCTGAGTTGATGCAGTTAGCTAAAACTAATAATATTGCTACTTTTATTGTAGGTCATG
TGACTAAGGAGGGAACATTAGCAGGCCCTCGAATGCTAGAACATATGGTGGATACGGTTCTTTATTTTGAAGGGGAGCGT
CATCACACGTTCCGTATTTTACGGGCAGTCAAAAACCGTTTTGGTTCGACCAATGAAATAGGTATCTTTGAAATGCAGTC
GGGTGGTCTAGTTGAAGTTTTGAATCCAAGTCAAGTTTTTCTTGAAGAACGTTTAGACGGTGCAACAGGATCTGCTATTG
TTGTTACTATGGAAGGAACAAGGCCTATTTTAGCAGAGGTTCAAGCTTTAGTGACTCCAACTGTTTTTGGAAATGCTAAG
CGCACAACCACAGGTCTTGACTTTAATCGTGTCAGTCTTATTATGGCAGTTCTAGAAAAACGTTGTGGTTTATTGCTTCA
AAATCAAGATGCCTATTTAAAATCAGCGGGTGGTGTTAAATTGGATGAACCAGCAATTGATTTAGCTGTTGCTGTAGCTA
TTGCTTCCAGTTACAAAGAAAAACCCACAAATCCAAAAGAATCATTTATTGGAGAAATTGGACTCACTGGTGAGATTCGT
CGCGTGACACGTATTGAGCAACGTATTAATGAGGCTAGTAAATTGGGCTTTACTAAAATTTACGCACCAAAGAATTCTCT
AGCTGGGATTGAAATACCTAAAGGCATTGATGTTATTGGTGTGACAACTGTTAGTCAGGTCTTAAAAGCAGTGTTTTCAA
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

87.859

99.78

0.877
  radA Streptococcus pneumoniae D39

87.859

99.78

0.877
  radA Streptococcus pneumoniae R6

87.859

99.78

0.877
  radA Streptococcus pneumoniae TIGR4

87.859

99.78

0.877
  radA Streptococcus mitis NCTC 12261

87.859

99.78

0.877
  radA Streptococcus mitis SK321

87.638

99.78

0.874
  radA Bacillus subtilis subsp. subtilis str. 168

62.914

99.78

0.628

Multiple sequence alignment