Detailed information NTG0326052

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: EGX80_RS06345
• Genome accession: NZ_CP033815
• Coordinates: 1192241..1193311 (+)
• Length: 356 a.a.
• NCBI ID: WP_002992231.1
• Uniprot ID: A0A5S4TWS5
• Organism: Streptococcus pyogenes strain FDAARGOS_514
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 1187241..1198311

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGX80_RS06330 (EGX80_06345)	amiA	1187769..1189739 (+)	1971	WP_002992224.1	peptide ABC transporter substrate-binding protein	Regulator
EGX80_RS06335 (EGX80_06350)	amiC	1189804..1191306 (+)	1503	WP_011054186.1	ABC transporter permease	Regulator
EGX80_RS06340 (EGX80_06355)	amiD	1191306..1192232 (+)	927	WP_011054187.1	oligopeptide ABC transporter permease OppC	Regulator
EGX80_RS06345 (EGX80_06360)	amiE	1192241..1193311 (+)	1071	WP_002992231.1	ABC transporter ATP-binding protein	Regulator
EGX80_RS06350 (EGX80_06365)	amiF	1193304..1194227 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
EGX80_RS10415 (EGX80_06370)	-	1194265..1194354 (-)	90	WP_109821088.1	IS3 family transposase	-
EGX80_RS10140	-	1194375..1194620 (-)	246	WP_030126956.1	hypothetical protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39680.74 Da
• Isoelectric Point: 5.4774

>NTDB_id=326052 EGX80_RS06345 WP_002992231.1 1192241..1193311(+) (amiE) [Streptococcus pyogenes strain FDAARGOS_514]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSNAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=326052 EGX80_RS06345 WP_002992231.1 1192241..1193311(+) (amiE) [Streptococcus pyogenes strain FDAARGOS_514]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATTCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTAATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A5S4TWS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537