Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FNE88_RS20300 Genome accession   NZ_CP041411
Coordinates   4121278..4121718 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain STEC719     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4116278..4126718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNE88_RS20280 (FNE88_20620) aroP 4117276..4118649 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  FNE88_RS20285 (FNE88_20625) ampE 4118692..4119546 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FNE88_RS20290 (FNE88_20630) ampD 4119543..4120094 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FNE88_RS20295 (FNE88_20635) nadC 4120182..4121075 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FNE88_RS20300 (FNE88_20640) pilA 4121278..4121718 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FNE88_RS20305 (FNE88_20645) pilB 4121728..4123113 (+) 1386 WP_001025149.1 type II secretion system protein GspE Machinery gene
  FNE88_RS20310 (FNE88_20650) hofC 4123103..4124305 (+) 1203 WP_000157234.1 protein transport protein HofC -
  FNE88_RS20315 (FNE88_20655) guaC 4124340..4125383 (-) 1044 WP_001217338.1 GMP reductase -
  FNE88_RS25905 - 4125539..4125583 (-) 45 WP_120795372.1 protein YacM -
  FNE88_RS20320 (FNE88_20660) coaE 4125608..4126228 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=325979 FNE88_RS20300 WP_000360904.1 4121278..4121718(+) (pilA) [Escherichia coli strain STEC719]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=325979 FNE88_RS20300 WP_000360904.1 4121278..4121718(+) (pilA) [Escherichia coli strain STEC719]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363