Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EGX95_RS18785 Genome accession   NZ_CP033795
Coordinates   3145841..3146275 (-) Length   144 a.a.
NCBI ID   WP_000811499.1    Uniprot ID   A0A1J9ZQC5
Organism   Bacillus sp. FDAARGOS_527     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3140841..3151275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGX95_RS18760 (EGX95_18750) - 3140916..3141703 (+) 788 Protein_3284 transposase -
  EGX95_RS18770 (EGX95_18760) - 3141863..3142420 (+) 558 WP_000062073.1 PadR family transcriptional regulator -
  EGX95_RS18775 (EGX95_18765) metE 3143089..3145377 (+) 2289 WP_001007591.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EGX95_RS18780 (EGX95_18770) comJ 3145415..3145804 (-) 390 WP_000424028.1 competence protein ComJ -
  EGX95_RS18785 (EGX95_18775) nucA/comI 3145841..3146275 (-) 435 WP_000811499.1 DNA-entry nuclease Machinery gene
  EGX95_RS18790 (EGX95_18780) - 3146512..3147261 (+) 750 WP_000388462.1 DUF3967 domain-containing protein -
  EGX95_RS18795 (EGX95_18785) kinB 3147312..3148586 (-) 1275 WP_000420386.1 sporulation sensor histidine kinase KinB -
  EGX95_RS18800 (EGX95_18790) - 3148896..3149096 (+) 201 WP_000929285.1 hypothetical protein -
  EGX95_RS18805 (EGX95_18795) - 3149220..3150377 (+) 1158 WP_000434567.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15996.35 Da        Isoelectric Point: 8.4331

>NTDB_id=325756 EGX95_RS18785 WP_000811499.1 3145841..3146275(-) (nucA/comI) [Bacillus sp. FDAARGOS_527]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=325756 EGX95_RS18785 WP_000811499.1 3145841..3146275(-) (nucA/comI) [Bacillus sp. FDAARGOS_527]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGATCGATACCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTGTGTACAATCGACCGTGGTGGAGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCGCACATCGAATATATAAGTCCAGCAGATAATCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTAAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J9ZQC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556

Multiple sequence alignment