Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EGX83_RS17945 Genome accession   NZ_CP033768
Coordinates   3745167..3745967 (+) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain FDAARGOS_533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3740167..3750967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGX83_RS17920 (EGX83_17915) - 3741077..3742375 (+) 1299 WP_001991191.1 glycosyltransferase family 4 protein -
  EGX83_RS17925 (EGX83_17920) - 3742400..3742951 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  EGX83_RS17930 (EGX83_17925) iscX 3743051..3743251 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  EGX83_RS17935 (EGX83_17930) ndk 3743365..3743796 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  EGX83_RS17940 (EGX83_17935) rlmN 3743919..3745151 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EGX83_RS17945 (EGX83_17940) pilF 3745167..3745967 (+) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EGX83_RS17950 (EGX83_17945) - 3745958..3746749 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  EGX83_RS17955 (EGX83_17950) ispG 3746771..3747886 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EGX83_RS17960 (EGX83_17955) hisS 3747883..3749175 (+) 1293 WP_000095262.1 histidine--tRNA ligase -
  EGX83_RS17965 (EGX83_17960) - 3749204..3749902 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=325581 EGX83_RS17945 WP_004738086.1 3745167..3745967(+) (pilF) [Acinetobacter baumannii strain FDAARGOS_533]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=325581 EGX83_RS17945 WP_004738086.1 3745167..3745967(+) (pilF) [Acinetobacter baumannii strain FDAARGOS_533]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment