Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FK544_RS20695 Genome accession   NZ_CP041304
Coordinates   3970031..3970471 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain MSHS 133     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3965031..3975471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK544_RS20675 (FK544_20660) aroP 3966032..3967402 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  FK544_RS20680 (FK544_20665) ampE 3967445..3968299 (-) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  FK544_RS20685 (FK544_20670) ampD 3968296..3968847 (-) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FK544_RS20690 (FK544_20675) nadC 3968935..3969828 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FK544_RS20695 (FK544_20680) pilA 3970031..3970471 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FK544_RS20700 (FK544_20685) pilB 3970481..3971866 (+) 1386 WP_001025165.1 type II secretion system protein GspE Machinery gene
  FK544_RS20705 (FK544_20690) hofC 3971856..3973058 (+) 1203 WP_000157293.1 protein transport protein HofC -
  FK544_RS20710 (FK544_20695) guaC 3973093..3974136 (-) 1044 WP_001217338.1 GMP reductase -
  FK544_RS25875 - 3974292..3974336 (-) 45 WP_120795372.1 protein YacM -
  FK544_RS20715 (FK544_20700) coaE 3974361..3974981 (+) 621 WP_001269512.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=325398 FK544_RS20695 WP_000360904.1 3970031..3970471(+) (pilA) [Escherichia coli strain MSHS 133]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=325398 FK544_RS20695 WP_000360904.1 3970031..3970471(+) (pilA) [Escherichia coli strain MSHS 133]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment