Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FK544_RS02815 Genome accession   NZ_CP041304
Coordinates   367986..369482 (+) Length   498 a.a.
NCBI ID   WP_001040613.1    Uniprot ID   -
Organism   Escherichia coli strain MSHS 133     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 362986..374482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK544_RS02785 (FK544_02780) tusA 363066..363311 (+) 246 WP_000130619.1 sulfurtransferase TusA -
  FK544_RS02790 (FK544_02785) zntA 363580..365778 (-) 2199 WP_000106587.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  FK544_RS02795 (FK544_02790) yhhN 365852..366478 (-) 627 WP_000964718.1 lysoplasmalogenase -
  FK544_RS02800 (FK544_02795) yhhM 366619..366978 (+) 360 WP_000042885.1 DUF2500 domain-containing protein -
  FK544_RS02805 (FK544_02800) yhhL 366981..367250 (-) 270 WP_001295207.1 DUF1145 family protein -
  FK544_RS02810 (FK544_02805) rsmD 367240..367836 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  FK544_RS02815 (FK544_02810) pilA 367986..369482 (+) 1497 WP_001040613.1 signal recognition particle-docking protein FtsY Machinery gene
  FK544_RS02820 (FK544_02815) ftsE 369485..370153 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  FK544_RS02825 (FK544_02820) ftsX 370146..371204 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  FK544_RS02830 (FK544_02825) rpoH 371449..372303 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  FK544_RS02835 (FK544_02830) livJ 372575..373678 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  FK544_RS02840 (FK544_02835) panM 373866..374249 (-) 384 WP_000778791.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54620.36 Da        Isoelectric Point: 4.1639

>NTDB_id=325347 FK544_RS02815 WP_001040613.1 367986..369482(+) (pilA) [Escherichia coli strain MSHS 133]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIAQAQEPAKASEHAVEEQPQAHTEAEAETFAADVVEVSEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=325347 FK544_RS02815 WP_001040613.1 367986..369482(+) (pilA) [Escherichia coli strain MSHS 133]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGCTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCTCTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAAGCGGTGATAGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCAACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAACCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTTTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGAGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378