Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   FK543_RS23610 Genome accession   NZ_CP041302
Coordinates   4653522..4655351 (-) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli strain MSHS 472     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4648522..4660351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK543_RS23585 (FK543_23580) bioP 4649240..4650139 (-) 900 WP_001196238.1 biotin transporter -
  FK543_RS23590 (FK543_23585) yigL 4650215..4651015 (-) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  FK543_RS23595 (FK543_23590) pldB 4651023..4652045 (-) 1023 WP_000487654.1 lysophospholipase L2 -
  FK543_RS23600 (FK543_23595) rhtB 4652156..4652776 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  FK543_RS23605 (FK543_23600) rhtC 4652838..4653458 (-) 621 WP_000928824.1 threonine export protein RhtC -
  FK543_RS23610 (FK543_23605) recQ 4653522..4655351 (-) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  FK543_RS23615 (FK543_23610) pldA 4655484..4656353 (-) 870 WP_001259700.1 phospholipase A -
  FK543_RS23620 (FK543_23615) yigI 4656518..4656985 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  FK543_RS23625 (FK543_23620) rarD 4657037..4657924 (+) 888 WP_000339103.1 EamA family transporter RarD -
  FK543_RS23630 (FK543_23625) - 4657971..4658270 (-) 300 WP_000526434.1 hypothetical protein -
  FK543_RS23640 (FK543_23635) - 4658894..4659046 (-) 153 Protein_4397 CorA family divalent cation transporter -
  FK543_RS23645 (FK543_23640) - 4659227..4660075 (+) 849 WP_001059703.1 YPO3983 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=325334 FK543_RS23610 WP_000035581.1 4653522..4655351(-) (recQ) [Escherichia coli strain MSHS 472]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=325334 FK543_RS23610 WP_000035581.1 4653522..4655351(-) (recQ) [Escherichia coli strain MSHS 472]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCTGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGTAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391

Multiple sequence alignment