Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FK543_RS19915 Genome accession   NZ_CP041302
Coordinates   3876032..3876472 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain MSHS 472     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3871032..3881472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK543_RS19895 (FK543_19890) aroP 3872030..3873403 (+) 1374 WP_072133621.1 aromatic amino acid transporter AroP -
  FK543_RS19900 (FK543_19895) ampE 3873446..3874300 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  FK543_RS19905 (FK543_19900) ampD 3874297..3874848 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FK543_RS19910 (FK543_19905) nadC 3874936..3875829 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FK543_RS19915 (FK543_19910) pilA 3876032..3876472 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FK543_RS19920 (FK543_19915) pilB 3876482..3877867 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  FK543_RS19925 (FK543_19920) hofC 3877857..3879059 (+) 1203 WP_000157236.1 protein transport protein HofC -
  FK543_RS19930 (FK543_19925) guaC 3879094..3880137 (-) 1044 WP_001217338.1 GMP reductase -
  FK543_RS24720 - 3880293..3880337 (-) 45 WP_120795372.1 protein YacM -
  FK543_RS19935 (FK543_19930) coaE 3880362..3880982 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=325320 FK543_RS19915 WP_000360895.1 3876032..3876472(+) (pilA) [Escherichia coli strain MSHS 472]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=325320 FK543_RS19915 WP_000360895.1 3876032..3876472(+) (pilA) [Escherichia coli strain MSHS 472]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363