Detailed information NTG0324489

Bioinformatically predicted

Overview

• Name: clpE
• Type: Regulator
• Locus tag: EGC57_RS06230
• Genome accession: NZ_CP033621
• Coordinates: 1203063..1205345 (+)
• Length: 760 a.a.
• NCBI ID: WP_002983882.1
• Uniprot ID: A0A6C2WI15
• Organism: Streptococcus pyogenes strain M75
• Function: repress competence development (at the early growth phase) (predicted from homology) Competence regulation

Genomic Context

Location: 1198063..1210345

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGC57_RS06210 (EGC57_06220)	-	1199430..1200839 (-)	1410	WP_129328322.1	deoxyribodipyrimidine photo-lyase	-
EGC57_RS06215 (EGC57_06225)	-	1200988..1201722 (-)	735	WP_010922488.1	amino acid ABC transporter ATP-binding protein	-
EGC57_RS06220 (EGC57_06230)	-	1201722..1202408 (-)	687	WP_011054708.1	amino acid ABC transporter permease	-
EGC57_RS06225 (EGC57_06235)	-	1202535..1202765 (-)	231	WP_002983885.1	YkuJ family protein	-
EGC57_RS06230 (EGC57_06240)	clpE	1203063..1205345 (+)	2283	WP_002983882.1	ATP-dependent Clp protease ATP-binding subunit	Regulator
EGC57_RS06235 (EGC57_06245)	-	1205473..1205928 (+)	456	WP_020905319.1	NUDIX hydrolase	-
EGC57_RS06240 (EGC57_06250)	-	1205979..1206281 (+)	303	WP_002983878.1	DUF1827 family protein	-
EGC57_RS06245 (EGC57_06255)	ileS	1206545..1209346 (-)	2802	WP_076639311.1	isoleucine--tRNA ligase	-

Sequence

Protein

• Length: 760 a.a.
• Molecular weight: 84718.88 Da
• Isoelectric Point: 5.0123

>NTDB_id=324489 EGC57_RS06230 WP_002983882.1 1203063..1205345(+) (clpE) [Streptococcus pyogenes strain M75]
MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSILNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLP
NTPPTQAGQNGNGGGRYGGNYNGQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRNRKDVILFIDEIHE
IVGAGSAGDGNMDAGNILKPALARGELQLVGATTLNEYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHH
VKYSPAAIEAAAHLSNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYERAAYFRDQ
ITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVNLANDLKAHVIGQDDAVDKIAKAIRRNRVGL
GTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPY
SLILLDEVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAREGRTSSVLGELSNFFS
PEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIHLDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAIT
DYYLEHPTEKQLRALMTNSENITIKAVKEGDSFLNEESLD

Nucleotide

• Length: 2283 bp

>NTDB_id=324489 EGC57_RS06230 WP_002983882.1 1203063..1205345(+) (clpE) [Streptococcus pyogenes strain M75]
ATGCTGTGTCAAAATTGTAATTTAAACGAATCTACTATTCATCTTTATACAAGTGTTAATGGAAAACAAAGACAGGTTGA
TCTCTGTCAAAATTGTTATCAAATCATGAAATCCGATCCTGCCAATTCTATTTTAAATGGCCTAACCCCAGGATATAGAG
CACAAGATAGATCCACAAGTCCTTTCTTTGATGACTTTTTTGGTGATTTGAATAATTTTAGAGCTTTTGGTAATCTTCCA
AATACCCCACCTACTCAGGCAGGGCAAAATGGAAATGGCGGAGGACGCTATGGTGGTAACTACAACGGACAACGACCTGC
TCAGCCACAAACACCAAATCAGCAAGCAAAGGGCTTATTAGAAGAGTTTGGGATTAATGTCACAGATATTGCAAGAAATG
GTAATATTGATCCTGTTATTGGTCGTGACGAAGAGATTACACGCGTTATCGAGATCCTCAACCGCCGTACTAAAAATAAT
CCTGTGCTAATTGGTGAGCCTGGAGTTGGTAAAACTGCTGTTGTAGAAGGTTTGGCTCAAAAAATTATTGATGGTACTGT
TCCTCAAAAACTCCAAGGCAAGCAAGTGATTCGTCTTGATGTGGTTAGCCTCGTTCAGGGAACAGGTATCCGTGGTCAGT
TTGAAGAGCGCATGCAAAAATTAATGGAAGAAATTCGCAATCGCAAGGATGTGATTCTCTTTATTGATGAAATTCATGAG
ATTGTCGGTGCTGGTTCTGCAGGAGACGGCAATATGGATGCTGGTAATATTTTAAAACCAGCCTTGGCCCGCGGTGAATT
GCAGTTGGTCGGCGCTACCACCTTAAATGAATATCGTATTATTGAAAAAGACGCTGCCTTAGAACGACGTATGCAACCCG
TTAAAGTTGATGAACCTTCTGTTGAGGAAACCATCACGATTTTAAAAGGTATCCAACCGAAATACGAAGACTATCATCAT
GTCAAATATAGCCCAGCCGCTATTGAAGCTGCCGCTCATTTATCTAACCGCTATATTCAAGACCGTTTTCTTCCTGATAA
GGCTATTGACCTTCTGGACGAAGCTGGTTCCAAAATGAATCTAACCCTCAACTTTGTTGATCCTAAAGAAATTGACAAAC
GTCTTATTGAAGCTGAGAATCTCAAGGCGCAAGCTACTCGAGACGAAGACTATGAACGCGCAGCTTATTTCCGCGATCAA
ATTACAAAATACAAAGAAATGCAGGCTCAAAAAGTCGATGAGCAAGATATTCCCATCATTACTGAAAAAACCATTGAAGC
TATTGTAGAGCAAAAAACTAATATTCCAGTTGGGGACTTAAAAGAAAAGGAACAGTCTCAACTCGTAAACTTAGCCAATG
ATCTGAAAGCACACGTGATCGGGCAAGACGATGCTGTTGATAAAATCGCTAAGGCTATTCGTCGTAACCGTGTGGGATTA
GGAACTCCAAATCGCCCTATTGGTTCTTTCCTATTCGTTGGACCGACTGGGGTTGGTAAAACAGAATTGTCTAAACAACT
AGCCATTGAACTCTTTGGTTCGACAAACAATATGATTCGCTTTGACATGTCCGAATACATGGAAAAACACGCTGTTGCCA
AATTAGTCGGGGCTCCTCCAGGTTATGTCGGCTATGAAGAAGCTGGACAGCTAACCGAACAAGTTCGTCGCAATCCATAT
TCACTTATTCTCTTAGATGAGGTGGAAAAGGCTCATCCTGACGTCATGCACATGTTCTTACAAGTTCTTGATGACGGTCG
CTTAACAGACGGTCAAGGGCGAACAGTCAGCTTCAAGGACACCATTATTATCATGACCTCTAATGCCGGAACAGGTAAAA
GCGAAGCTTCTGTCGGATTTGGTGCTGCTAGAGAAGGACGGACAAGTTCTGTCCTTGGTGAATTAAGCAACTTCTTCAGC
CCAGAGTTTATGAATCGTTTCGATGGTATTATTGAATTCAAAGCTTTATCTAAAGAACATTTGCTGCATATCGTTGATTT
AATGCTGGAAGATGTTAATGAGCGCTTAGGCTATAATGGGATTCATCTTGATGTGACACAAAAGGTCAAAGAAAAATTAG
TAGATTTAGGCTATGATCCTAAAATGGGCGCTCGGCCGCTCCGTCGTACTATCCAAGATTATATCGAAGATGCCATTACA
GACTATTATTTAGAACACCCAACTGAAAAACAGTTACGTGCACTGATGACAAACAGTGAAAACATCACGATTAAAGCTGT
TAAAGAGGGAGATTCTTTTCTTAACGAAGAGTCTCTAGATTAA

Domains

Predicted by InterproScan.

• AAA lid domain (AAA_lid_9) (300-397)

• ATPase family associated with various cellular activities (AAA) (AAA) (162-281)

• C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small) (656-729)

• AAA domain (Cdc48 subfamily) (AAA_2) (485-648)

3D structure

• AlphaFold DB: A0A6C2WI15

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
clpE	Streptococcus mutans UA159	84.759	98.421	0.834
clpE	Streptococcus pneumoniae Rx1	82.69	98.816	0.817
clpE	Streptococcus pneumoniae D39	82.69	98.816	0.817
clpE	Streptococcus pneumoniae R6	82.69	98.816	0.817
clpE	Streptococcus pneumoniae TIGR4	82.557	98.816	0.816
clpC	Lactococcus lactis subsp. cremoris KW2	75.164	100	0.753
clpC	Bacillus subtilis subsp. subtilis str. 168	51.593	86.711	0.447
clpC	Lactococcus lactis subsp. lactis strain DGCC12653	46	85.526	0.393
clpC	Streptococcus pneumoniae Rx1	47.84	82.237	0.393
clpC	Streptococcus pneumoniae D39	47.84	82.237	0.393
clpC	Streptococcus pneumoniae TIGR4	47.84	82.237	0.393
clpC	Streptococcus mutans UA159	47.573	81.316	0.387
clpC	Streptococcus thermophilus LMD-9	47.115	82.105	0.387
clpC	Streptococcus thermophilus LMG 18311	46.795	82.105	0.384