Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EF513_RS05135 Genome accession   NZ_CP033611
Coordinates   1108777..1110159 (-) Length   460 a.a.
NCBI ID   WP_125216340.1    Uniprot ID   A0A3S8T3E7
Organism   Rickettsiales endosymbiont of Stachyamoeba lipophora strain RICK01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1103777..1115159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EF513_RS05105 (EF513_05150) - 1104431..1105063 (+) 633 WP_125216334.1 helix-turn-helix domain-containing protein -
  EF513_RS05110 (EF513_05155) - 1105307..1105528 (-) 222 WP_125216335.1 hypothetical protein -
  EF513_RS05115 (EF513_05160) - 1105518..1105961 (-) 444 WP_125216336.1 hypothetical protein -
  EF513_RS05120 (EF513_05165) - 1106048..1106527 (-) 480 WP_164503832.1 transcription antitermination factor NusB -
  EF513_RS05125 (EF513_05170) ribH 1106533..1106970 (-) 438 WP_125216338.1 6,7-dimethyl-8-ribityllumazine synthase -
  EF513_RS08120 (EF513_05175) ribB 1107197..1108351 (-) 1155 WP_125216339.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
  EF513_RS05135 (EF513_05180) radA 1108777..1110159 (-) 1383 WP_125216340.1 DNA repair protein RadA Machinery gene
  EF513_RS05140 (EF513_05185) queF 1110163..1110978 (-) 816 WP_125216341.1 NADPH-dependent 7-cyano-7-deazaguanine reductase QueF -
  EF513_RS05145 (EF513_05190) - 1111328..1112560 (+) 1233 WP_125216342.1 hypothetical protein -
  EF513_RS05150 (EF513_05195) rlmB 1112768..1113484 (-) 717 WP_125216343.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EF513_RS05165 (EF513_05210) tuf 1113954..1115144 (+) 1191 WP_125216271.1 elongation factor Tu -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 50650.14 Da        Isoelectric Point: 7.7572

>NTDB_id=324406 EF513_RS05135 WP_125216340.1 1108777..1110159(-) (radA) [Rickettsiales endosymbiont of Stachyamoeba lipophora strain RICK01]
MAKKSSQYICQECGSVSQKWQGKCPDCGAWNSIIEEAINDNTSFYASKVNKLNNNQIQFFDINQEIAEQTRINSYYQELN
QVLGGGFVPGSAVLIGGDPGIGKSTLLLQITAKLSSHNHKVIYVSGEESASQITLRAKRLNINNSGLKLLTATNLDLILN
KVKDEQLDFLIIDSIQTVFLEAIGSAPGTVSQVRACSHELIRLAKERNITLILVGHVTKEGQIAGPKVLEHMVDTVLYFE
GESSYQYRLLRSVKNRFGSINEIGVFEMHGSGLIEVTNPSSLFIGERSQNISGSSIYAGIEGTRPIFAEIQALVAPTFMA
TPRRAVVGWDNNRLAMIIAVLATRFGLNLTDKELYLNVMSGLKISEPAMDLSAAAALISAATNQPIIENSVFIGEVGLSG
EVRTVQHLETRVKESVKLGFKNIITPKFTKDNELRDTLTKQQIQLHEIKHIKQLKQIIFS

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=324406 EF513_RS05135 WP_125216340.1 1108777..1110159(-) (radA) [Rickettsiales endosymbiont of Stachyamoeba lipophora strain RICK01]
ATGGCAAAAAAAAGCAGCCAATATATCTGCCAAGAATGCGGCAGTGTTAGCCAAAAATGGCAAGGTAAATGCCCTGATTG
TGGCGCCTGGAACAGCATAATCGAAGAAGCAATTAACGACAATACTAGCTTTTATGCTAGCAAAGTTAATAAACTTAATA
ATAATCAAATCCAATTTTTTGATATAAACCAAGAAATAGCTGAGCAAACCAGAATTAATTCATATTACCAAGAGCTAAAT
CAAGTATTAGGAGGTGGGTTTGTACCTGGTTCTGCAGTATTAATTGGGGGTGATCCAGGCATTGGTAAATCCACTTTATT
GCTACAAATAACTGCTAAATTATCTAGCCATAATCATAAAGTTATTTATGTATCAGGTGAAGAATCCGCCTCTCAAATTA
CCCTAAGAGCAAAAAGACTTAACATCAACAATTCTGGGCTTAAGCTACTTACTGCCACTAATTTGGACCTTATTCTAAAC
AAGGTGAAAGATGAACAGCTGGATTTTTTAATCATCGACTCTATTCAAACCGTATTTTTAGAGGCAATTGGTTCTGCCCC
AGGTACAGTTTCACAAGTTAGGGCCTGCTCACATGAACTTATACGTCTTGCTAAAGAACGTAATATTACTCTTATTTTAG
TAGGACATGTTACTAAAGAAGGACAAATTGCTGGTCCTAAAGTATTAGAACATATGGTAGATACCGTACTTTATTTTGAG
GGTGAAAGCAGCTATCAATACCGACTGCTTAGATCGGTTAAAAACCGCTTTGGCAGTATTAATGAAATTGGTGTATTTGA
AATGCATGGCAGCGGGCTCATTGAGGTAACCAATCCCTCTTCGCTATTTATTGGCGAAAGAAGCCAAAATATTAGCGGCA
GCAGTATTTATGCTGGCATTGAAGGAACTAGGCCAATATTTGCTGAAATTCAAGCATTAGTTGCCCCCACCTTTATGGCC
ACTCCTAGACGTGCAGTAGTAGGTTGGGATAACAACCGACTTGCAATGATCATTGCAGTACTTGCCACACGCTTTGGACT
CAACTTAACTGATAAAGAACTTTATTTAAATGTAATGTCAGGACTTAAAATTAGCGAGCCAGCCATGGATCTATCAGCAG
CAGCTGCTTTAATTTCTGCAGCCACCAACCAACCGATCATAGAAAACTCAGTATTTATAGGAGAAGTTGGCCTTTCAGGT
GAAGTGCGTACGGTTCAACATCTTGAAACCAGAGTTAAAGAATCCGTAAAACTTGGTTTTAAAAATATAATTACCCCTAA
ATTTACTAAAGATAATGAATTAAGAGATACCCTAACTAAACAGCAAATTCAACTTCATGAAATTAAGCATATTAAGCAGC
TAAAGCAGATTATATTTAGTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-221)

Subunit ChlI of Mg-chelatase (ChlI)

(345-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S8T3E7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.835

94.783

0.491
  radA Streptococcus mitis NCTC 12261

47.527

100

0.48
  radA Streptococcus pneumoniae Rx1

47.527

100

0.48
  radA Streptococcus pneumoniae D39

47.527

100

0.48
  radA Streptococcus pneumoniae R6

47.527

100

0.48
  radA Streptococcus pneumoniae TIGR4

47.527

100

0.48
  radA Streptococcus mitis SK321

47.527

100

0.48

Multiple sequence alignment