Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EFD52_RS09710 Genome accession   NZ_CP033606
Coordinates   1991749..1993110 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis strain IL6288     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1986749..1998110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD52_RS09675 (EFD52_09675) - 1986803..1987126 (-) 324 WP_010906291.1 DUF1048 domain-containing protein -
  EFD52_RS09680 (EFD52_09680) - 1987113..1987460 (-) 348 WP_010906292.1 DUF1048 domain-containing protein -
  EFD52_RS09685 (EFD52_09685) - 1987447..1987809 (-) 363 WP_003129889.1 PadR family transcriptional regulator -
  EFD52_RS09690 (EFD52_09690) - 1987929..1988558 (-) 630 WP_003129888.1 hypothetical protein -
  EFD52_RS09695 (EFD52_09695) - 1988623..1989708 (-) 1086 WP_010906293.1 PIN/TRAM domain-containing protein -
  EFD52_RS09700 (EFD52_09700) - 1989899..1990846 (-) 948 WP_010906258.1 IS30 family transposase -
  EFD52_RS09705 (EFD52_09705) - 1991019..1991705 (+) 687 WP_003130565.1 DUF1275 family protein -
  EFD52_RS09710 (EFD52_09710) radA 1991749..1993110 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  EFD52_RS09715 (EFD52_09715) - 1993272..1993499 (-) 228 WP_010906294.1 heavy-metal-associated domain-containing protein -
  EFD52_RS09730 (EFD52_09730) rplQ 1994314..1994694 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  EFD52_RS09735 (EFD52_09735) - 1994713..1995651 (-) 939 WP_010906296.1 DNA-directed RNA polymerase subunit alpha -
  EFD52_RS09740 (EFD52_09740) rpsK 1995699..1996082 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  EFD52_RS09745 (EFD52_09745) rpsM 1996103..1996468 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  EFD52_RS09750 (EFD52_09750) rpmJ 1996486..1996602 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  EFD52_RS09755 (EFD52_09755) infA 1996656..1996874 (-) 219 WP_003130554.1 translation initiation factor IF-1 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=324334 EFD52_RS09710 WP_003130563.1 1991749..1993110(-) (radA) [Lactococcus lactis strain IL6288]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=324334 EFD52_RS09710 WP_003130563.1 1991749..1993110(-) (radA) [Lactococcus lactis strain IL6288]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTCGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGTCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGCTCAACTAATGAAATTGGAATCTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment