Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EB239_RS13995 Genome accession   NZ_CP033580
Coordinates   2795505..2796866 (-) Length   453 a.a.
NCBI ID   WP_003870692.1    Uniprot ID   M8CUC3
Organism   Thermoanaerobacter ethanolicus JW 200     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2790505..2801866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB239_RS13970 ispF 2791130..2791627 (-) 498 WP_003870689.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EB239_RS13975 - 2791620..2792864 (-) 1245 WP_003870690.1 lipase/acyltransferase domain-containing protein -
  EB239_RS13980 ispD 2792931..2793614 (-) 684 WP_003868427.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EB239_RS13985 - 2793697..2794800 (-) 1104 WP_003868426.1 PIN/TRAM domain-containing protein -
  EB239_RS13990 - 2795083..2795478 (+) 396 WP_003870691.1 hypothetical protein -
  EB239_RS13995 radA 2795505..2796866 (-) 1362 WP_003870692.1 DNA repair protein RadA Machinery gene
  EB239_RS14000 - 2796913..2798178 (-) 1266 WP_003870693.1 Nramp family divalent metal transporter -
  EB239_RS14005 - 2798274..2798738 (-) 465 WP_003870694.1 metal-dependent transcriptional regulator -
  EB239_RS14010 ltrA 2798866..2800278 (-) 1413 WP_129545166.1 group II intron reverse transcriptase/maturase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50972.81 Da        Isoelectric Point: 5.3387

>NTDB_id=324150 EB239_RS13995 WP_003870692.1 2795505..2796866(-) (radA) [Thermoanaerobacter ethanolicus JW 200]
MAKSTTKFVCRECGFESSKWMGRCPNCDSWNSFEEETIEDSKNFRKNKDRSQIFLLEDVTFSEEERIKTGIEEFDRVLGG
GIVKGSLVLIGGDPGIGKSTLLLQVAGNISDNRKVLYVSGEESIQQIKLRADRILKEKQQIYLLCETNIEEIERKIDENK
PDFVIIDSIQTMYTEESETIPGSVSQVRQVTQRLMEIAKQKDISIFLIGHVTKEGAIAGPKVLEHMVDTVLYFEGDRTQS
FRILRAVKNRFGATHEIGVFDMEEDGLCEISNPSEFILSNKPKDVSGTAVVCSIQGTRPILMEVQSLICKTTFGVPRRMA
TGFDYNRFILLLAVIEKRLGINLSQFDAYVNVAGGMKIQEPAADLGIIASSLSGYFNIPIPEDVCFIGEVGLTGEVRGVS
NIDKRIGEAQKMGFSQVFIPEMKTKEIKKFENIKIIKVRDIKEIMDFLRKIKQ

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=324150 EB239_RS13995 WP_003870692.1 2795505..2796866(-) (radA) [Thermoanaerobacter ethanolicus JW 200]
ATGGCCAAATCAACTACAAAATTTGTGTGTAGAGAGTGTGGCTTTGAAAGCAGTAAATGGATGGGAAGATGTCCTAATTG
TGACAGCTGGAATTCTTTTGAGGAAGAAACGATAGAGGATTCAAAAAATTTTAGGAAAAATAAAGATAGATCGCAAATAT
TTCTTCTCGAAGATGTAACTTTTTCAGAAGAAGAAAGAATAAAGACAGGGATTGAAGAGTTTGACAGGGTTTTAGGTGGC
GGAATTGTAAAAGGTTCTCTTGTTTTAATAGGTGGTGACCCAGGAATTGGGAAGTCTACTTTGCTATTACAAGTAGCAGG
TAATATTTCTGATAACAGAAAGGTACTTTATGTGTCAGGAGAGGAATCAATTCAACAGATTAAACTGAGGGCAGATAGAA
TTTTGAAAGAAAAGCAACAAATATATCTCTTGTGCGAAACAAATATAGAAGAGATAGAGAGAAAAATTGATGAGAATAAG
CCGGATTTTGTTATTATTGATTCTATACAGACAATGTACACTGAGGAAAGTGAGACAATACCAGGAAGTGTGTCTCAGGT
AAGGCAAGTGACTCAAAGGCTGATGGAGATTGCAAAACAAAAAGACATAAGTATTTTTTTGATAGGACATGTTACAAAAG
AAGGGGCAATAGCAGGCCCTAAAGTTTTGGAACATATGGTAGACACAGTTTTGTATTTTGAAGGGGATAGGACCCAGTCT
TTTAGGATATTGCGGGCTGTAAAAAACCGTTTTGGCGCAACTCACGAAATAGGAGTTTTTGATATGGAGGAGGATGGGCT
TTGTGAGATAAGCAATCCTTCTGAATTTATTCTCTCAAATAAGCCGAAAGATGTTTCAGGGACTGCTGTTGTATGTTCTA
TTCAAGGGACGAGGCCAATTTTAATGGAAGTGCAGTCTTTGATATGTAAGACTACTTTTGGAGTGCCGCGAAGAATGGCC
ACTGGATTTGACTACAATCGTTTTATATTGCTTTTGGCAGTTATAGAAAAAAGACTGGGCATAAATCTTTCGCAATTTGA
TGCATATGTAAATGTGGCTGGAGGAATGAAAATTCAAGAGCCAGCTGCTGACCTTGGAATAATTGCTTCATCTCTTTCTG
GATATTTTAATATACCTATTCCTGAAGATGTTTGTTTTATAGGAGAGGTGGGATTGACGGGAGAAGTGAGAGGAGTCAGC
AATATAGATAAACGAATAGGTGAAGCACAAAAGATGGGATTTTCTCAAGTTTTTATTCCTGAGATGAAAACAAAAGAAAT
AAAAAAGTTTGAGAATATAAAAATTATTAAGGTAAGAGATATAAAAGAAATTATGGATTTTTTAAGAAAGATAAAACAAT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-215)

Subunit ChlI of Mg-chelatase (ChlI)

(340-421)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M8CUC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.319

99.779

0.532
  radA Streptococcus mitis NCTC 12261

51.562

98.896

0.51
  radA Streptococcus pneumoniae Rx1

51.562

98.896

0.51
  radA Streptococcus pneumoniae D39

51.562

98.896

0.51
  radA Streptococcus pneumoniae R6

51.562

98.896

0.51
  radA Streptococcus pneumoniae TIGR4

51.562

98.896

0.51
  radA Streptococcus mitis SK321

51.562

98.896

0.51

Multiple sequence alignment