Detailed information NTG0324053

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: EEB07_RS08105
• Genome accession: NZ_CP033576
• Coordinates: 1550354..1551499 (+)
• Length: 381 a.a.
• NCBI ID: WP_094247421.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain NY12-2
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1545354..1556499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EEB07_RS08090 (EEB07_08090)	-	1547101..1547832 (-)	732	Protein_1569	MFS transporter	-
EEB07_RS08095 (EEB07_08095)	argS	1547967..1549637 (-)	1671	WP_025649385.1	arginine--tRNA ligase	-
EEB07_RS08100 (EEB07_08100)	-	1549634..1550062 (-)	429	WP_003151069.1	DUF1934 family protein	-
EEB07_RS08105 (EEB07_08105)	rapF	1550354..1551499 (+)	1146	WP_094247421.1	Rap family tetratricopeptide repeat protein	Regulator
EEB07_RS08110 (EEB07_08110)	-	1551483..1551602 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
EEB07_RS08115 (EEB07_08115)	speB	1551725..1552597 (-)	873	WP_003151054.1	agmatinase	-
EEB07_RS08120 (EEB07_08120)	speE	1552657..1553487 (-)	831	WP_003151052.1	spermidine synthase	-
EEB07_RS08125 (EEB07_08125)	-	1553687..1555759 (+)	2073	WP_123117494.1	transglycosylase domain-containing protein	-
EEB07_RS08130 (EEB07_08130)	-	1555784..1556215 (-)	432	WP_017418704.1	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45782.29 Da
• Isoelectric Point: 5.0722

>NTDB_id=324053 EEB07_RS08105 WP_094247421.1 1550354..1551499(+) (rapF) [Bacillus velezensis strain NY12-2]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSELLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDEKKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLENIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLESKLMYADIEELALDVAKYYYTKEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=324053 EEB07_RS08105 WP_094247421.1 1550354..1551499(+) (rapF) [Bacillus velezensis strain NY12-2]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGAGCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGAAAAAAAATACAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGAACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGCCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAGAAAATATAGATAATGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTGGAATATTTATATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAGTAAACTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACAAAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAAGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	69.816	100	0.698
rapC	Bacillus subtilis subsp. subtilis str. 168	53.684	99.738	0.535