Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   EEB07_RS00525 Genome accession   NZ_CP033576
Coordinates   75998..76606 (+) Length   202 a.a.
NCBI ID   WP_003153521.1    Uniprot ID   -
Organism   Bacillus velezensis strain NY12-2     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 70998..81606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEB07_RS00485 (EEB07_00485) - 72051..72557 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  EEB07_RS00490 (EEB07_00490) - 72696..73109 (+) 414 WP_007612169.1 hypothetical protein -
  EEB07_RS00495 (EEB07_00495) - 73139..73573 (-) 435 WP_123117288.1 YppG family protein -
  EEB07_RS00500 (EEB07_00500) - 73760..73942 (+) 183 WP_003153532.1 YppF family protein -
  EEB07_RS00505 (EEB07_00505) - 73981..74349 (-) 369 WP_003153529.1 YppE family protein -
  EEB07_RS00510 (EEB07_00510) - 74395..74640 (-) 246 WP_039063257.1 DUF5446 family protein -
  EEB07_RS00515 (EEB07_00515) sspM 74846..74950 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  EEB07_RS00520 (EEB07_00520) - 74994..75956 (-) 963 WP_063636681.1 DUF2515 domain-containing protein -
  EEB07_RS00525 (EEB07_00525) recU 75998..76606 (+) 609 WP_003153521.1 Holliday junction resolvase RecU Machinery gene
  EEB07_RS00530 (EEB07_00530) - 76645..79422 (+) 2778 WP_123117289.1 PBP1A family penicillin-binding protein -
  EEB07_RS00535 (EEB07_00535) - 79500..79991 (-) 492 WP_012117839.1 YpoC family protein -
  EEB07_RS00540 (EEB07_00540) nth 79988..80647 (-) 660 WP_007612187.1 endonuclease III -
  EEB07_RS00545 (EEB07_00545) dnaD 80666..81364 (-) 699 WP_017418023.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23680.82 Da        Isoelectric Point: 9.6872

>NTDB_id=324018 EEB07_RS00525 WP_003153521.1 75998..76606(+) (recU) [Bacillus velezensis strain NY12-2]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=324018 EEB07_RS00525 WP_003153521.1 75998..76606(+) (recU) [Bacillus velezensis strain NY12-2]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTCGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AAGAACGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.955

98.515

0.896

Multiple sequence alignment