Detailed information NTG0323979

Bioinformatically predicted

Overview

• Name: chiS
• Type: Regulator
• Locus tag: EEL44_RS18255
• Genome accession: NZ_CP033515
• Coordinates: 2529007..2532540 (+)
• Length: 1177 a.a.
• NCBI ID: WP_001883436.1
• Uniprot ID: -
• Organism: Vibrio cholerae strain E4
• Function: chitin sensor (predicted from homology) Competence regulation

Genomic Context

Location: 2524007..2537540

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EEL44_RS18235 (EEL44_18235)	erpA	2524586..2524927 (-)	342	WP_000005741.1	iron-sulfur cluster insertion protein ErpA	-
EEL44_RS18240 (EEL44_18240)	hemL	2525211..2526509 (+)	1299	WP_000167257.1	glutamate-1-semialdehyde 2,1-aminomutase	-
EEL44_RS18245 (EEL44_18245)	-	2526763..2527848 (+)	1086	WP_001290813.1	AI-2E family transporter	-
EEL44_RS18250 (EEL44_18250)	rsmC	2527921..2528943 (+)	1023	WP_001183380.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
EEL44_RS18255 (EEL44_18255)	chiS	2529007..2532540 (+)	3534	WP_001883436.1	two-component system sensor histidine kinase ChiS	Regulator
EEL44_RS18265 (EEL44_18265)	-	2533451..2535121 (+)	1671	WP_000880698.1	ABC transporter substrate-binding protein	-
EEL44_RS18270 (EEL44_18270)	-	2535291..2536277 (+)	987	WP_000540337.1	ABC transporter permease	-
EEL44_RS18275 (EEL44_18275)	-	2536280..2537305 (+)	1026	WP_000646270.1	ABC transporter permease	-

Sequence

Protein

• Length: 1177 a.a.
• Molecular weight: 133134.30 Da
• Isoelectric Point: 6.8941

>NTDB_id=323979 EEL44_RS18255 WP_001883436.1 2529007..2532540(+) (chiS) [Vibrio cholerae strain E4]
MRLCPRFSLDLLVKVKKLVNFVTLGFSCLSPTHAWRSPQSFTESNTLIMFRFYRKQKFKRLQNTLMAAFLALSIIPLTIT
ALFFLHSHSKDLEQQSTSYLVSVRDNKQQQVIDYMMAKESEVMGFVRSELAYASGGRFYGLVNAFQRLDVSIEAAREHAQ
QRYITGSGDQIKTSVLPQSSSYVGSERYRLLHKRYHWAYLELLKRSDFDDILLVDIDGNVVYSIYKYDNFGTNLLTGKYQ
DTNLGHTFKRLEQTVNEQRKTNEDFTPVVISDFVQEDGKQYAWLGAPIIQQGYLHSYAMFRLPSNAITKLIAEGSSNPSM
QTILVGQDHRSRTLTSAEIAVEKSKAVVDLALSGKRAVGTYTNTDGEQIIAAYAPINLKNIHWALVVELPEKEAFARVRQ
LEKLFVFAMLTAIVLVVIASHYLSNFITSPLLKLTWAAERVSAGDLDEAMINTERKDEIGRLAVSFERMQRSIREKISLI
KSQNKELESNLLIIRKQNDELQLANKLKDEFLATTSHELRTPLHGMIGIAEALISGANGPISAAHKYQLDIIISSGQRLA
TLVDDLLDYHKMRYGALDIQRCAVDLSSATRLVLELSHHLLGKKTLRIINQVSEQPVWVSADPQRLEQVLYNLIGNAIKY
TSEGKIVISATYIDDKVRVQVVDTGQGIPAEQLEHIFEPLIQAGHDASRYRQGAGLGLSISRQLIELMHGTLYVSSQPMV
GTTFSFTLPLASEEEIAQARLTELPHFQAPEVLDSELPEQSNLPENEHGPLLLVADDEPVNLRVLDSFLRLEGYRVHTAQ
DGHQVLEAIKREKPELVLLDIMMPGMSGYQVCEKLRQTYDHAELPIIMLTALNQSDDRVRGFEAGANDYLSKPFNKQELA
ARIVAHLTASKAELRRIENAQLQKELKHRAMVEASLLETQGRLLEQLESAPEAILCVKEGNKVRFANEAAARLFRRTPEQ
LKRSNAEELIAPKFLNIEQEHYCGNIDVYVDDVRQHLSADVLRLPQGSGLQAMYIFNVGGSVNAARIHNLETAVEALSSY
AFEGDKDKLQKLKELGGEFTRLADKASGEYQSKQDLMRAVLVEAMTSALNYWERVSGQSKFTFAEQSGLWRVYLDRSTLQ
TRTLDKYLRIETLPKTPRWRTVLNSLDYILEHCKEAGPERTHIEMQRDKLQKLLTSE

Nucleotide

• Length: 3534 bp

>NTDB_id=323979 EEL44_RS18255 WP_001883436.1 2529007..2532540(+) (chiS) [Vibrio cholerae strain E4]
TTGCGTCTATGCCCACGCTTTAGCTTGGACTTACTTGTCAAAGTAAAAAAACTCGTTAATTTCGTTACTTTAGGTTTTTC
TTGTCTATCACCCACTCATGCTTGGCGATCGCCGCAGAGTTTCACCGAAAGTAACACCTTAATTATGTTTAGGTTCTATC
GAAAACAAAAATTCAAACGCCTGCAAAATACCTTGATGGCTGCGTTTTTGGCCCTCAGCATTATTCCTTTGACCATTACT
GCGTTGTTTTTCCTACATTCACACAGTAAAGATCTGGAGCAGCAGAGCACTTCTTACCTCGTTTCTGTTCGGGATAATAA
ACAGCAACAGGTCATCGACTACATGATGGCCAAAGAGTCGGAAGTGATGGGTTTTGTTCGCTCCGAGCTGGCTTATGCCA
GTGGCGGGCGTTTTTATGGTCTGGTGAATGCTTTCCAGCGTTTAGACGTCAGTATTGAAGCGGCTCGTGAACATGCTCAG
CAGCGCTATATCACCGGATCGGGTGACCAAATAAAAACCTCGGTGCTCCCCCAATCAAGCAGCTACGTGGGCAGTGAACG
CTATCGACTGCTGCATAAACGCTACCACTGGGCTTACTTAGAACTGCTGAAACGGTCGGACTTTGATGACATTTTATTGG
TCGATATTGATGGCAACGTCGTTTATTCGATTTATAAATACGACAATTTTGGCACCAACTTATTAACGGGCAAATACCAA
GACACCAATCTAGGCCATACCTTCAAGCGTTTAGAACAAACGGTCAATGAACAGCGCAAAACCAACGAAGATTTCACCCC
TGTCGTGATTTCTGATTTCGTACAAGAGGATGGTAAACAATACGCTTGGCTTGGCGCACCGATTATTCAGCAAGGCTACT
TGCATAGTTACGCGATGTTCCGCTTACCAAGCAATGCGATCACCAAACTCATCGCAGAAGGCAGTAGCAATCCTTCGATG
CAAACCATTCTAGTGGGACAAGATCACCGCTCAAGAACCTTAACTTCCGCCGAAATTGCGGTTGAAAAAAGTAAAGCTGT
GGTCGATTTAGCCTTATCGGGCAAACGCGCCGTAGGCACTTACACCAATACCGATGGTGAACAGATCATCGCGGCCTACG
CACCGATCAATTTGAAAAATATCCATTGGGCTCTGGTGGTTGAACTGCCAGAGAAAGAAGCGTTTGCTCGCGTTCGTCAG
TTAGAGAAACTGTTTGTTTTTGCGATGTTGACGGCTATCGTCTTAGTGGTCATCGCCTCGCATTACCTTTCCAATTTCAT
TACCTCACCGCTTTTGAAGCTGACATGGGCAGCCGAACGTGTTTCGGCAGGCGATCTTGATGAAGCCATGATCAATACCG
AACGCAAAGATGAAATTGGCCGCTTGGCGGTCAGTTTTGAGCGTATGCAGCGTTCCATTCGCGAAAAAATATCGCTGATC
AAATCGCAAAATAAAGAGTTAGAGAGCAACTTACTGATCATTCGTAAGCAAAACGATGAGTTACAGCTAGCGAATAAGCT
AAAAGATGAGTTCCTCGCGACCACATCACATGAATTACGCACCCCATTGCATGGCATGATTGGTATTGCCGAAGCCCTGA
TCTCGGGGGCAAATGGCCCGATTTCAGCAGCGCACAAGTATCAACTCGACATCATTATCAGCAGTGGGCAGCGCTTGGCG
ACTCTGGTTGATGATCTACTCGATTACCACAAAATGCGCTATGGCGCGCTGGATATTCAAAGGTGTGCCGTCGATTTATC
GAGCGCTACGCGCTTAGTCTTGGAGCTTTCTCACCATTTACTTGGCAAGAAAACGCTGCGCATTATTAATCAAGTCTCAG
AACAACCGGTCTGGGTTTCTGCGGATCCTCAACGCTTAGAGCAAGTCCTGTATAACTTGATCGGCAATGCCATTAAGTAC
ACTTCCGAAGGCAAAATTGTTATCTCCGCAACCTATATTGACGATAAAGTGCGCGTGCAGGTGGTGGATACAGGTCAAGG
GATTCCGGCAGAACAACTGGAGCACATCTTTGAGCCATTGATTCAAGCGGGCCATGATGCGAGTCGCTATCGCCAAGGCG
CAGGACTCGGGCTTTCCATCAGTCGTCAGTTAATCGAATTGATGCACGGCACTCTCTACGTAAGTAGTCAGCCGATGGTT
GGCACTACCTTTAGCTTCACGCTGCCTCTGGCGAGCGAAGAAGAGATTGCTCAAGCGCGCTTGACCGAACTCCCCCACTT
CCAAGCACCGGAAGTGTTGGATTCTGAACTGCCCGAGCAAAGCAACTTACCCGAAAATGAACATGGCCCACTTCTGTTGG
TCGCCGATGATGAACCAGTCAACCTGCGTGTGCTGGACAGCTTTTTACGTCTTGAAGGTTATCGCGTACATACCGCGCAA
GATGGCCATCAAGTACTTGAAGCAATTAAACGTGAAAAACCTGAATTGGTGTTACTCGACATCATGATGCCAGGCATGAG
CGGTTATCAAGTGTGTGAAAAACTTCGACAAACTTACGACCACGCTGAACTGCCTATTATTATGCTGACGGCTCTCAACC
AGTCTGATGACCGGGTACGAGGGTTTGAAGCTGGAGCCAATGACTACTTATCCAAACCATTCAACAAACAAGAGCTGGCC
GCGCGCATTGTCGCTCACCTCACGGCGAGCAAAGCCGAACTGCGCCGCATTGAGAATGCCCAACTGCAAAAAGAGTTGAA
ACATCGCGCTATGGTAGAAGCGAGCCTATTGGAAACCCAAGGCCGATTATTGGAACAGTTAGAATCTGCGCCAGAAGCGA
TTTTGTGCGTTAAAGAGGGCAATAAAGTACGTTTTGCTAACGAAGCGGCAGCACGTTTATTTAGACGTACCCCTGAGCAG
CTCAAACGCTCCAACGCCGAAGAACTGATTGCTCCTAAATTCTTGAATATTGAGCAAGAGCATTACTGCGGCAATATTGA
TGTTTACGTAGATGATGTGCGCCAGCACCTCTCTGCCGATGTCCTTCGCTTGCCGCAAGGTTCTGGCTTACAAGCGATGT
ACATTTTTAATGTCGGCGGCAGCGTCAATGCCGCGCGGATTCATAACCTCGAAACCGCGGTAGAAGCCCTGTCTAGCTAT
GCCTTCGAAGGCGATAAAGACAAGCTGCAAAAACTCAAGGAACTGGGTGGTGAGTTTACTCGTCTTGCAGATAAAGCCTC
GGGCGAGTATCAATCGAAACAAGATTTGATGCGTGCCGTGTTAGTCGAAGCCATGACCAGTGCCTTGAACTATTGGGAAC
GAGTCTCAGGGCAAAGCAAGTTCACGTTTGCCGAACAAAGTGGCTTGTGGCGCGTTTATCTTGACCGCAGCACCCTACAA
ACTCGTACCCTAGACAAATACTTACGAATTGAGACACTGCCTAAAACACCGCGTTGGCGAACCGTACTGAACTCGCTCGA
CTACATTCTTGAGCATTGCAAAGAAGCAGGCCCTGAACGCACTCACATCGAAATGCAGCGCGATAAATTGCAAAAACTCC
TGACCAGTGAATAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (622-731)

• Response regulator receiver domain (Response_reg) (773-883)

• His Kinase A (phospho-acceptor) domain (HisKA) (508-573)

• PAS domain (PAS_8) (927-961)

• HAMP domain (HAMP) (423-475)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
chiS	Vibrio cholerae strain A1552	100	100	1