Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EBB51_RS12465 Genome accession   NZ_CP033465
Coordinates   2595471..2596826 (-) Length   451 a.a.
NCBI ID   WP_123054754.1    Uniprot ID   -
Organism   Clostridium sp. JN-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2590471..2601826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBB51_RS12445 ispD 2591657..2592352 (-) 696 WP_123054750.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EBB51_RS12450 - 2592354..2593460 (-) 1107 WP_123054751.1 PIN/TRAM domain-containing protein -
  EBB51_RS13720 - 2593642..2593782 (-) 141 WP_190285287.1 hypothetical protein -
  EBB51_RS12455 - 2593930..2594343 (+) 414 WP_123054752.1 DUF1573 domain-containing protein -
  EBB51_RS12460 disA 2594397..2595455 (-) 1059 WP_123054753.1 DNA integrity scanning diadenylate cyclase DisA -
  EBB51_RS12465 radA 2595471..2596826 (-) 1356 WP_123054754.1 DNA repair protein RadA Machinery gene
  EBB51_RS12470 - 2597019..2599466 (-) 2448 WP_123054755.1 ATP-dependent Clp protease ATP-binding subunit -
  EBB51_RS12475 - 2599486..2600517 (-) 1032 WP_123054756.1 protein arginine kinase -
  EBB51_RS12480 - 2600530..2601048 (-) 519 WP_123054757.1 UvrB/UvrC motif-containing protein -
  EBB51_RS12485 - 2601076..2601540 (-) 465 WP_123054758.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 49563.19 Da        Isoelectric Point: 8.2106

>NTDB_id=323888 EBB51_RS12465 WP_123054754.1 2595471..2596826(-) (radA) [Clostridium sp. JN-1]
MAKNKSIFVCQECGYESLRWLGKCPNCNTWNSMVEEAKEEKSSSKLSTALDSVPRSITSIKSGEYERLDTGISELNRVLG
GGVVKGSLTLISGAPGIGKSTLLLQTANNIANKYGKVLYVSGEESEEQIKMRGDRLNSLSENLYIISETNMEKIEEHIKN
TNPIFVVIDSIQTLFKQSMTSAPGSVSQVRQNSNDIMRIGKTKNIPFFIVAHITKQGELAGPRVLEHMVDTVLSFEGERT
EEFRILRTIKNRFGNTSEIGVFEMSQEGLRQISNPSAAFLEETGFQKEGSIVIGIIEGTRPILVEIQALVTETKAVIPRR
TAVGVDSSRLSLILAVLEKKLKVYFYNCDVYVNVVGGLNIDGTFGDLGLALALLSSVKSKAISLERLIVVGEIGLTGEVR
PITFCDRLINEAEKMGFKNAIIPFRNKGKIKNKNIDVIGVSSLVEAINKVF

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=323888 EBB51_RS12465 WP_123054754.1 2595471..2596826(-) (radA) [Clostridium sp. JN-1]
ATGGCAAAAAACAAAAGCATTTTTGTTTGTCAGGAATGTGGATATGAATCGTTAAGATGGCTTGGTAAGTGCCCTAATTG
TAATACATGGAACAGTATGGTTGAAGAAGCAAAAGAGGAAAAAAGCTCTTCTAAATTGAGTACTGCATTAGACAGTGTAC
CGCGCAGCATAACAAGTATAAAATCTGGAGAATATGAGAGACTTGATACTGGAATATCTGAGTTAAATAGAGTGTTAGGT
GGGGGGGTAGTTAAAGGATCACTTACATTGATATCAGGTGCACCAGGTATAGGAAAGTCAACGTTACTTCTACAGACAGC
AAACAATATTGCCAATAAATATGGTAAGGTATTATATGTATCAGGAGAAGAATCTGAGGAACAGATAAAGATGAGGGGAG
ATAGATTAAATTCTCTATCAGAAAATCTTTACATAATTTCTGAAACCAATATGGAAAAAATCGAGGAACATATAAAAAAT
ACAAATCCAATATTTGTTGTAATTGACTCAATACAAACGCTTTTTAAACAGTCTATGACTTCAGCACCGGGAAGTGTATC
TCAAGTACGACAAAATTCTAACGATATTATGCGTATAGGCAAAACAAAAAATATACCATTTTTTATAGTAGCACATATAA
CAAAACAAGGAGAATTAGCAGGACCAAGGGTGCTAGAACATATGGTAGATACTGTACTTTCTTTTGAAGGCGAAAGGACT
GAAGAATTCAGAATACTTAGAACCATAAAAAATCGTTTTGGTAATACTAGTGAAATTGGTGTATTTGAGATGTCCCAAGA
AGGATTAAGGCAAATATCAAATCCATCAGCTGCATTCCTAGAGGAAACAGGATTTCAAAAAGAGGGCTCTATTGTAATAG
GGATAATTGAAGGAACAAGACCAATATTAGTTGAAATACAAGCTCTTGTAACTGAAACTAAAGCAGTCATACCAAGAAGA
ACAGCTGTAGGAGTTGACAGTTCAAGATTGAGTTTAATATTAGCAGTTTTAGAAAAAAAACTGAAAGTCTACTTTTACAA
TTGTGATGTTTATGTTAATGTAGTAGGTGGGCTCAATATAGATGGAACTTTTGGAGACTTGGGGTTGGCATTGGCTCTTT
TGTCCAGTGTTAAATCAAAGGCAATATCACTTGAAAGGCTTATTGTAGTTGGAGAAATAGGACTCACAGGAGAGGTAAGA
CCTATTACCTTTTGTGACAGACTTATAAATGAGGCAGAAAAGATGGGGTTTAAAAATGCTATAATACCATTTAGAAATAA
GGGTAAAATAAAAAATAAAAATATTGATGTAATTGGGGTATCTTCTTTGGTAGAGGCAATAAATAAAGTTTTTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-217)

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.656

100

0.519
  radA Streptococcus mitis NCTC 12261

48.458

100

0.488
  radA Streptococcus pneumoniae Rx1

48.458

100

0.488
  radA Streptococcus pneumoniae D39

48.458

100

0.488
  radA Streptococcus pneumoniae R6

48.458

100

0.488
  radA Streptococcus pneumoniae TIGR4

48.458

100

0.488
  radA Streptococcus mitis SK321

48.238

100

0.486

Multiple sequence alignment