Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EC605_RS05715 Genome accession   NZ_CP033426
Coordinates   1136898..1138274 (+) Length   458 a.a.
NCBI ID   WP_005720413.1    Uniprot ID   A0A2A7RGN6
Organism   Lactobacillus crispatus strain CO3MRSI1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1131898..1143274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EC605_RS05690 - 1132776..1133180 (-) 405 WP_005720409.1 hypothetical protein -
  EC605_RS05695 pepC 1133320..1134669 (-) 1350 WP_005720410.1 aminopeptidase C -
  EC605_RS05700 - 1134737..1135036 (-) 300 WP_005721602.1 hypothetical protein -
  EC605_RS05705 - 1135143..1136177 (+) 1035 WP_005729050.1 IS30 family transposase -
  EC605_RS05710 - 1136347..1136898 (+) 552 WP_005720412.1 dUTP diphosphatase -
  EC605_RS05715 radA 1136898..1138274 (+) 1377 WP_005720413.1 DNA repair protein RadA Machinery gene
  EC605_RS05720 gltX 1138352..1139851 (+) 1500 WP_005720415.1 glutamate--tRNA ligase -
  EC605_RS05725 cysS 1139942..1141375 (+) 1434 WP_005720416.1 cysteine--tRNA ligase -
  EC605_RS05730 - 1141368..1141811 (+) 444 WP_005720417.1 mini-ribonuclease III -
  EC605_RS05735 rlmB 1141798..1142553 (+) 756 WP_005720418.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EC605_RS05740 - 1142687..1143232 (+) 546 WP_005720419.1 DNA-directed RNA polymerase subunit sigma -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50214.83 Da        Isoelectric Point: 7.1405

>NTDB_id=323682 EC605_RS05715 WP_005720413.1 1136898..1138274(+) (radA) [Lactobacillus crispatus strain CO3MRSI1]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVKLDKIKAEKEERIVTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMAVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFIPRHNMYSGLKDHGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=323682 EC605_RS05715 WP_005720413.1 1136898..1138274(+) (radA) [Lactobacillus crispatus strain CO3MRSI1]
ATGGCTAGAGTAAAAACACAATATAAGTGTCGTTCTTGCGGCTATATTTCGGCAAGTTATTTAGGCCGCTGTCCTAACTG
TGGTGCCTGGAATCAATTTGAAAAAGAAACTGAAGAAGTGCAAAAGCGTTCTACTAAGGCAACTGCTAGTCGTTTGATTC
AGAAGACTGGATTAAATGAACCGGTAAAGCTGGATAAGATTAAAGCTGAAAAAGAAGAACGAATTGTCACTAAATCTGAG
GAATTAAACCGCGTTTTAGGTGGTGGTATTGTACCAGGCTCACTAGTTTTAATTGGTGGAGATCCCGGAATTGGTAAGTC
AACCTTGATGTTACAAATTATGAGTGACTTATCTGAAAAATATAAGGTGCTTTACGTTTCGGGAGAAGAATCAGCTAACC
AAATCAAATTGCGGGCTGACCGACTGGGTGTAGGTCAAAGTAATATGCTCTTATATCCAGAGACTGACATGCATGATATT
CGCGAACAAATCAATGATGTTAAGCCTGACTTTGTTGTAATCGACTCCATTCAAACTATGAATGAACCAAGTCTTGATTC
GATGACTGGCTCTGCCTCACAGGTTCGTGAAGTAACAAGTGAATTAATGAAAATTGCCAAGATGGATGCCATTACAGTTT
TTGTTATCGGCCACGTTACAAAAGAGGGTGCGATTGCTGGGCCTAAGATCTTGGAACATATGGTGGATACCGTTCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGAATTTTGCATTCTGTTAAAAACCGTTTTGGAGCAGCAAACGAAATTGGAAT
GTTTGAAATGGTTAATGAAGGCTTAAGAGAGGTAACTAATCCATCATCAATTTTTCTGGACCAAAGATTACCTAATTCTA
CTGGCTCTGCTGTGGTGGTATCGCTTGAAGGAACTAGGCCACTTTTAGCAGAAATTCAGGCTTTGGTTACTCCGACAGCT
TTTGGCTATGCTAAAAGGACCACGTCAGGTATTAGTTTTAACAAAGCTTCATTGCTGCTAGCGGTGCTTGAAAAGCGGGG
AAACTTAATGTTGCAGAATCAAGATGTCTATTTGACTGCAACTGGTGGGATTAAATTGAATGAACCAGCAATTGATTTAG
CAATTGCCATGGCAGTTGCATCCAGTTATACCGATAAAGAAATTTCGCCGACAGATTGTTTTGTAGGCGAAGTGGGCTTA
ACTGGTGAAGTTCGCCGAGTTGATAAAATCGATGCACGAGTAAAAGAAGCGGCTAAAGTAGGGTTTAAGCGGATCTTTAT
TCCACGGCATAATATGTATTCTGGATTAAAAGATCATGGCATTGAAGTAATTCCCGTTTCGAGTATTCCCCAAGCTTTAA
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A7RGN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.773

100

0.559

Multiple sequence alignment