Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EC605_RS02215 Genome accession   NZ_CP033426
Coordinates   456237..458033 (+) Length   598 a.a.
NCBI ID   WP_005718172.1    Uniprot ID   A0A135YP31
Organism   Lactobacillus crispatus strain CO3MRSI1     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 451237..463033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EC605_RS02190 - 451479..452030 (+) 552 WP_005722451.1 GNAT family protein -
  EC605_RS02195 - 452520..452903 (+) 384 WP_005718176.1 PBECR4 domain-containing protein -
  EC605_RS02200 - 452900..453589 (-) 690 WP_005718174.1 DUF554 domain-containing protein -
  EC605_RS02205 - 453654..454892 (-) 1239 WP_005718173.1 LCP family protein -
  EC605_RS02210 - 455009..456043 (-) 1035 WP_005728483.1 IS30 family transposase -
  EC605_RS02215 pepF 456237..458033 (+) 1797 WP_005718172.1 oligoendopeptidase F Regulator
  EC605_RS02220 - 458185..458400 (+) 216 WP_005723825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  EC605_RS02225 - 458404..458811 (+) 408 WP_005723824.1 type II toxin-antitoxin system death-on-curing family toxin -
  EC605_RS02230 - 458925..459200 (-) 276 WP_005723823.1 bacteriocin immunity protein -
  EC605_RS02235 - 459204..459422 (-) 219 WP_005726358.1 TIGR04139 family peptide modification target -
  EC605_RS02240 - 459596..459892 (-) 297 WP_005725464.1 bacteriocin immunity protein -
  EC605_RS02245 - 459908..460168 (-) 261 WP_005726357.1 thioredoxin domain-containing protein -
  EC605_RS02250 - 460355..461533 (+) 1179 WP_005727388.1 IS256 family transposase -
  EC605_RS02255 - 461596..462084 (-) 489 Protein_426 response regulator -
  EC605_RS02260 - 462088..462996 (-) 909 WP_005729075.1 GHKL domain-containing protein -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68032.88 Da        Isoelectric Point: 4.6146

>NTDB_id=323674 EC605_RS02215 WP_005718172.1 456237..458033(+) (pepF) [Lactobacillus crispatus strain CO3MRSI1]
MAIPNRNEVPEELKWDLTRIFKNDEEWEQAYAAAQEKIDQLGELKGTLAKSGKDLYEGLTKILAVKREVENIYVYATMSS
DVDTANSHYLGYVSRVQSLANQFEAATSFINPEILSIPSDKLAEFKQAEPRLKNYAHYLEMITNKRPHTLPAEQEKLIAD
AGDALGVSENTFNVLTNSDMEYGYVQDDDGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYATYSQFQNSLASTLSGVVKK
HNYNAKAHKYDSARQAALAENGVPEAVYDTLIQEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGKPSLSYNFEEA
KEVAKKALKPLGEDYLKHVDYIFNNRVIDPIESKNKVTGAYSGGAYDTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFLHEADANGEPLTA
DTLDDVYGQINQHYYGDSVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTPAERDAYLGFLKAGSSDY
PTEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFESLIEK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=323674 EC605_RS02215 WP_005718172.1 456237..458033(+) (pepF) [Lactobacillus crispatus strain CO3MRSI1]
ATGGCGATACCAAACAGAAATGAAGTCCCAGAAGAACTTAAGTGGGACTTGACCCGAATTTTTAAAAATGATGAAGAATG
GGAACAGGCCTATGCAGCGGCCCAAGAAAAAATCGACCAACTTGGTGAATTAAAAGGTACATTAGCAAAATCTGGCAAAG
ATTTGTACGAAGGCTTAACTAAGATTCTGGCCGTTAAGCGCGAGGTGGAAAATATTTATGTTTATGCCACCATGTCTAGC
GATGTGGATACTGCCAATTCACATTATTTAGGCTATGTCAGCCGGGTGCAAAGCTTAGCTAACCAATTCGAAGCCGCTAC
TAGTTTTATTAACCCAGAAATCTTGAGCATCCCTAGTGATAAACTAGCTGAATTTAAGCAAGCTGAGCCAAGACTAAAGA
ATTATGCTCACTACTTGGAAATGATTACTAATAAGCGACCTCATACCCTGCCAGCTGAACAAGAAAAGTTAATCGCTGAT
GCTGGAGATGCTTTAGGCGTTTCCGAAAATACTTTTAACGTTTTAACTAACTCTGACATGGAATACGGCTACGTCCAAGA
TGACGATGGCAACATGGAACAACTATCTGACGGTTTGTACTCATTATTAATTCAATCGCAAAACCGTGACGTCAGAAAAG
GTGCCTTCGACACTCTCTATGCTACGTACAGCCAATTCCAGAATTCGCTGGCTTCAACCTTGTCCGGCGTAGTGAAAAAA
CACAACTATAACGCTAAAGCGCACAAATATGACTCTGCCCGTCAAGCAGCACTGGCTGAAAACGGCGTACCTGAAGCAGT
TTACGATACTTTAATTCAAGAAGTTGATTCTCACCTTGACCTGCTCCATCGTTACGTTGCCTTGCGCAAGAAAATTTTAG
GCTTAAAAGATTTGCAAATGTGGGACATGTACGTGCCGCTAACTGGGAAGCCTTCTCTTTCTTATAACTTTGAAGAAGCT
AAGGAAGTTGCTAAAAAGGCATTGAAGCCTTTGGGCGAAGACTACTTGAAGCATGTTGACTATATTTTTAATAATCGTGT
CATCGACCCAATAGAATCTAAAAACAAGGTAACTGGTGCCTACTCCGGTGGTGCTTATGATACCGATCCTTATGAGTTGC
TCAACTGGGAAGATAACATCGATTCACTTTATACTTTAGTCCACGAAACTGGTCACTCCGTTCACTCCTGGTACACTCGC
AATACTCAACCTTACGTTTACGGCGACTATCCAATCTTTGTGGCTGAAATTGCTTCAACTACTAATGAAAATATTTTGAC
CGAATATTTCTTAGACCACATTACCGATCCTAAGACGCGGGCCTTTATCTTAAATTACTACCTTGATTCATTTAAAGGCA
CTTTATTCCGTCAAACTCAATTTGCCGTGTTTGAACAATTCTTGCACGAAGCCGATGCTAATGGAGAACCACTGACCGCT
GACACTTTAGACGACGTTTACGGTCAAATTAATCAGCATTATTACGGCGATAGCGTTGAACCAGGCGGCGATATTGCCCT
TGAATGGTCACGAATTCCACACTTCTACTACAATTTCTATGTGTACCAATATGCGACTGGCTTTGCGGCTGCAACAGCAT
TAGCAAACAAGGTGGTTCATGGAACGCCTGCTGAGAGGGATGCCTACCTTGGCTTTCTTAAAGCTGGTTCAAGTGATTAC
CCAACTGAGATCATGAAGCGTGCTGGCGTAGACATGACTAAGCCTGACTACTTAGAAGATGCCTTCAAGACTTTTGAAAA
ACGGTTGAACGAATTTGAAAGCTTGATTGAGAAATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-583)

Oligopeptidase F (Peptidase_M3_N)

(113-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A135YP31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.515

99.331

0.512

Multiple sequence alignment