Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FHY70_RS07930 Genome accession   NZ_CP040886
Coordinates   1258632..1259072 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain K71-77     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1253632..1264072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHY70_RS07910 (FHY70_07910) aroP 1254630..1256003 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FHY70_RS07915 (FHY70_07915) ampE 1256046..1256900 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FHY70_RS07920 (FHY70_07920) ampD 1256897..1257448 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FHY70_RS07925 (FHY70_07925) nadC 1257536..1258429 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FHY70_RS07930 (FHY70_07930) pilA 1258632..1259072 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FHY70_RS07935 (FHY70_07935) pilB 1259082..1260467 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  FHY70_RS07940 (FHY70_07940) hofC 1260457..1261659 (+) 1203 WP_060504081.1 protein transport protein HofC -
  FHY70_RS07945 (FHY70_07945) guaC 1261694..1262737 (-) 1044 WP_001217342.1 GMP reductase -
  FHY70_RS25855 - 1262893..1262937 (-) 45 WP_120795372.1 protein YacM -
  FHY70_RS07950 (FHY70_07950) coaE 1262962..1263582 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=323646 FHY70_RS07930 WP_000360895.1 1258632..1259072(+) (pilA) [Escherichia coli strain K71-77]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=323646 FHY70_RS07930 WP_000360895.1 1258632..1259072(+) (pilA) [Escherichia coli strain K71-77]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363