Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D5285_RS14250 Genome accession   NZ_CP033389
Coordinates   2812196..2812825 (-) Length   209 a.a.
NCBI ID   WP_020452274.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CBMAI 1303     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2807196..2817825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5285_RS14230 (D5285_14230) holA 2807755..2808780 (-) 1026 WP_020452271.1 DNA polymerase III subunit delta -
  D5285_RS14235 (D5285_14235) - 2809010..2809141 (+) 132 WP_003183686.1 YqzM family protein -
  D5285_RS14240 (D5285_14240) - 2809166..2811553 (-) 2388 WP_041817260.1 DNA internalization-related competence protein ComEC/Rec2 -
  D5285_RS14245 (D5285_14245) - 2811558..2812127 (-) 570 WP_020452273.1 ComE operon protein 2 -
  D5285_RS14250 (D5285_14250) comEA 2812196..2812825 (-) 630 WP_020452274.1 helix-hairpin-helix domain-containing protein Machinery gene
  D5285_RS14255 (D5285_14255) comER 2812908..2813729 (+) 822 WP_020452275.1 late competence protein ComER -
  D5285_RS14260 (D5285_14260) - 2813799..2814554 (-) 756 WP_020452276.1 class I SAM-dependent methyltransferase -
  D5285_RS14265 (D5285_14265) rsfS 2814551..2814907 (-) 357 WP_020452277.1 ribosome silencing factor -
  D5285_RS14270 (D5285_14270) yqeK 2814921..2815484 (-) 564 WP_020452278.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D5285_RS14275 (D5285_14275) - 2815474..2816043 (-) 570 WP_020452279.1 nicotinate-nucleotide adenylyltransferase -
  D5285_RS14280 (D5285_14280) yhbY 2816062..2816352 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  D5285_RS14285 (D5285_14285) aroE 2816346..2817182 (-) 837 WP_020452280.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22592.49 Da        Isoelectric Point: 4.6439

>NTDB_id=323575 D5285_RS14250 WP_020452274.1 2812196..2812825(-) (comEA) [Bacillus paralicheniformis strain CBMAI 1303]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSDISIPEEASAQTFDKKEEVKLKKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSGDIVNINTASSEELQT
IPGIGPSKAEAVIEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=323575 D5285_RS14250 WP_020452274.1 2812196..2812825(-) (comEA) [Bacillus paralicheniformis strain CBMAI 1303]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGAGTGGCACTCGTCTTGATCATCAGCGCGGCCTTCAT
GCTGTTAAGCGGAAAGCGCGAAACTTCATCCGACATTTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAAGTGAAGCTGAAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
AATTAATCTGGCGGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAGGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAATCGAATACCGTGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(146-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.804

100

0.541
  comEA Staphylococcus aureus MW2

35.909

100

0.378
  comEA Staphylococcus aureus N315

35.455

100

0.373

Multiple sequence alignment