Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   D5285_RS14065 Genome accession   NZ_CP033389
Coordinates   2777330..2778100 (-) Length   256 a.a.
NCBI ID   WP_020452240.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CBMAI 1303     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2772330..2783100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5285_RS14045 (D5285_14045) - 2772405..2773217 (-) 813 WP_020452237.1 pyruvate, water dikinase regulatory protein -
  D5285_RS14050 (D5285_14050) - 2773243..2773881 (-) 639 WP_025804968.1 helix-turn-helix transcriptional regulator -
  D5285_RS14055 (D5285_14055) glyS 2774079..2776118 (-) 2040 WP_020452238.1 glycine--tRNA ligase subunit beta -
  D5285_RS14060 (D5285_14060) glyQ 2776111..2776998 (-) 888 WP_020452239.1 glycine--tRNA ligase subunit alpha -
  D5285_RS14065 (D5285_14065) recO 2777330..2778100 (-) 771 WP_020452240.1 DNA repair protein RecO Machinery gene
  D5285_RS14070 (D5285_14070) - 2778138..2778281 (-) 144 WP_003183626.1 YqzL family protein -
  D5285_RS14075 (D5285_14075) era 2778443..2779348 (-) 906 WP_020452241.1 GTPase Era -
  D5285_RS14080 (D5285_14080) - 2779329..2779739 (-) 411 WP_020452242.1 cytidine deaminase -
  D5285_RS14085 (D5285_14085) - 2779872..2780252 (-) 381 WP_020452243.1 diacylglycerol kinase family protein -
  D5285_RS14090 (D5285_14090) ybeY 2780230..2780706 (-) 477 WP_020452244.1 rRNA maturation RNase YbeY -
  D5285_RS14095 (D5285_14095) - 2780703..2782757 (-) 2055 WP_228119640.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29527.17 Da        Isoelectric Point: 8.3124

>NTDB_id=323573 D5285_RS14065 WP_020452240.1 2777330..2778100(-) (recO) [Bacillus paralicheniformis strain CBMAI 1303]
MLTKCEGIVLRTNDYGETNKIITLLTREHGKIGVLARGAKKPNSRLSAISQPFLYASFLFRKSSGLGTLEQGEMIESMRH
IREDLFLTAYAAYITELIDKGTEEKKPNPFLFELLLQSMRRLDDGTDPDIIANLIEVKMLSVMGLYPELDECVHCKSKDG
TFHFSIRDNGFICHRCFAKDPYRIPLSPASARLLRLFFYFDLSRLGNVSVKQETKDELKRVIQTYYDEYSGIYLKSRRFM
EQMDNMKKMLGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=323573 D5285_RS14065 WP_020452240.1 2777330..2778100(-) (recO) [Bacillus paralicheniformis strain CBMAI 1303]
ATGCTGACGAAATGTGAAGGTATCGTCCTTCGTACAAACGACTATGGTGAAACAAATAAAATCATTACGCTGTTGACGAG
AGAACACGGGAAAATCGGTGTGCTGGCAAGGGGCGCCAAAAAGCCGAACAGCCGCTTGTCAGCGATTTCCCAGCCGTTCC
TTTATGCTTCTTTTTTATTCCGCAAATCATCCGGTCTCGGCACATTGGAGCAGGGCGAGATGATCGAAAGCATGAGGCAT
ATACGCGAGGATCTGTTTTTAACAGCATACGCGGCCTACATCACTGAATTAATCGACAAGGGAACGGAGGAAAAGAAACC
GAATCCGTTTTTATTTGAGCTGCTTCTTCAGTCGATGAGGAGGCTCGATGACGGTACAGATCCCGACATTATCGCCAACC
TTATCGAAGTTAAAATGCTCAGCGTGATGGGGCTGTATCCGGAGCTGGATGAATGTGTACATTGCAAAAGCAAAGACGGC
ACATTTCATTTTTCGATCCGCGACAACGGCTTTATCTGCCACCGCTGTTTTGCGAAAGATCCATACAGGATACCGCTGTC
TCCCGCATCCGCCAGGCTGTTGAGGCTGTTTTTTTATTTTGACCTGTCAAGGCTCGGAAATGTCTCGGTTAAACAGGAGA
CGAAGGATGAGCTGAAAAGGGTCATACAGACGTACTATGACGAGTACTCAGGGATCTACCTGAAATCGCGGCGCTTCATG
GAACAAATGGACAATATGAAAAAGATGCTGGGCGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

77.344

100

0.773

Multiple sequence alignment