Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FGE23_RS13035 Genome accession   NZ_CP040672
Coordinates   2566298..2567287 (+) Length   329 a.a.
NCBI ID   WP_003154301.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain X030     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2561298..2572287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGE23_RS13020 acpP 2561495..2561728 (+) 234 WP_003154310.1 acyl carrier protein -
  FGE23_RS13025 rncS 2561867..2562616 (+) 750 WP_003154303.1 ribonuclease III -
  FGE23_RS13030 smc 2562718..2566278 (+) 3561 WP_146276760.1 chromosome segregation protein SMC -
  FGE23_RS13035 pilA 2566298..2567287 (+) 990 WP_003154301.1 signal recognition particle-docking protein FtsY Machinery gene
  FGE23_RS13040 - 2567371..2568141 (+) 771 WP_070081816.1 phosphotransferase family protein -
  FGE23_RS13045 - 2568261..2568593 (+) 333 WP_003154299.1 putative DNA-binding protein -
  FGE23_RS13050 ffh 2568607..2569947 (+) 1341 WP_003154298.1 signal recognition particle protein -
  FGE23_RS13055 rpsP 2570053..2570325 (+) 273 WP_003154296.1 30S ribosomal protein S16 -
  FGE23_RS13060 - 2570325..2570570 (+) 246 WP_007611392.1 KH domain-containing protein -
  FGE23_RS13065 - 2570661..2571047 (+) 387 WP_003154291.1 YlqD family protein -
  FGE23_RS13070 rimM 2571052..2571576 (+) 525 WP_003154290.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 36096.28 Da        Isoelectric Point: 5.0098

>NTDB_id=323097 FGE23_RS13035 WP_003154301.1 2566298..2567287(+) (pilA) [Bacillus amyloliquefaciens strain X030]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFSNKVNELVSRYRKVDEDFFEELEEVLIGADVGFTTVMELIDELKKEV
KLKNIQDPQEVQSVISEKLVDIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKLKNEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQAAGSDPAAVIYDAVHAAKARGADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQRFDAESYVYGLF
SDLVEKAED

Nucleotide


Download         Length: 990 bp        

>NTDB_id=323097 FGE23_RS13035 WP_003154301.1 2566298..2567287(+) (pilA) [Bacillus amyloliquefaciens strain X030]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACGAAACAGACAGACTCCGTCTCTGAAAAATTTAAAGATGGTCTTGA
AAAAACAAGAAATTCCTTCTCAAATAAAGTGAACGAACTCGTCTCCCGCTACCGGAAAGTCGACGAGGATTTCTTCGAGG
AGCTTGAGGAAGTGCTGATTGGCGCTGACGTAGGTTTTACAACCGTCATGGAGCTGATTGATGAGCTCAAAAAAGAAGTG
AAGCTGAAAAACATCCAGGATCCTCAGGAAGTGCAGTCCGTCATTTCTGAAAAGCTTGTCGACATTTATAACAGCGGCGA
CGAACAGATTTCTGAATTAAATATTCAGGACGGGCGCCTGAACGTTATTCTGCTTGTCGGGGTAAACGGTGTCGGAAAAA
CGACAACGATCGGAAAACTTGCCCATAAGTTGAAGAATGAAGGCAAATCCGTTGTGCTCGCTGCCGGAGACACCTTCAGA
GCCGGGGCGATTGAACAGCTTGAAGTGTGGGGAGAGCGTACAGGCGTGCCTGTCATTAAGCAGGCTGCCGGAAGCGACCC
TGCGGCGGTTATTTATGATGCCGTACACGCGGCTAAAGCAAGAGGAGCGGATGTGCTGATTTGTGATACGGCCGGCCGCC
TTCAGAATAAAGTTAACTTAATGAAAGAGCTTGAAAAGGTCAAACGCGTCATCGAACGCGAAGTGCCTGAGGCTCCGCAT
GAGGTTCTCCTCGCGCTTGACGCGACGACAGGTCAGAATGCGATGGCGCAGGCGAAAGAATTCTCCAAAGCGACGAATGT
GACGGGTATCGCATTAACGAAGCTTGACGGCACCGCAAAAGGCGGTATCGTTCTGGCGATCCGCAATGAGCTTCACATCC
CCGTGAAACTGGTCGGCCTTGGAGAAAAGGTTGACGATCTCCAGCGGTTTGATGCAGAATCTTACGTATACGGTCTCTTT
TCGGATTTAGTTGAAAAAGCCGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(121-320)

SRP54-type protein, helical bundle domain (SRP54_N)

(33-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

45.333

91.185

0.413

Multiple sequence alignment