Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D9Y32_RS14420 Genome accession   NZ_CP033218
Coordinates   2803701..2804330 (+) Length   209 a.a.
NCBI ID   WP_003183693.1    Uniprot ID   Q8VQ77
Organism   Bacillus licheniformis strain TCCC 11148     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2798701..2809330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9Y32_RS14385 (D9Y32_14410) aroE 2799343..2800179 (+) 837 WP_003183706.1 shikimate dehydrogenase -
  D9Y32_RS14390 (D9Y32_14415) yhbY 2800173..2800463 (+) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  D9Y32_RS14395 (D9Y32_14420) - 2800482..2801051 (+) 570 WP_003183702.1 nicotinate-nucleotide adenylyltransferase -
  D9Y32_RS14400 (D9Y32_14425) yqeK 2801041..2801604 (+) 564 WP_003183701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D9Y32_RS14405 (D9Y32_14430) rsfS 2801618..2801974 (+) 357 WP_003183699.1 ribosome silencing factor -
  D9Y32_RS14410 (D9Y32_14435) - 2801971..2802726 (+) 756 WP_011198132.1 class I SAM-dependent methyltransferase -
  D9Y32_RS14415 (D9Y32_14440) comER 2802796..2803617 (-) 822 WP_009327864.1 late competence protein ComER -
  D9Y32_RS14420 (D9Y32_14445) comEA 2803701..2804330 (+) 630 WP_003183693.1 helix-hairpin-helix domain-containing protein Machinery gene
  D9Y32_RS14425 (D9Y32_14450) - 2804399..2804968 (+) 570 WP_003183690.1 ComE operon protein 2 -
  D9Y32_RS14430 (D9Y32_14455) - 2804972..2807359 (+) 2388 WP_142782509.1 DNA internalization-related competence protein ComEC/Rec2 -
  D9Y32_RS14435 (D9Y32_14460) - 2807384..2807515 (-) 132 WP_003183686.1 YqzM family protein -
  D9Y32_RS14440 (D9Y32_14465) holA 2807745..2808788 (+) 1044 WP_025804972.1 DNA polymerase III subunit delta -
  D9Y32_RS14445 (D9Y32_14470) rpsT 2808850..2809116 (-) 267 WP_003183682.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22468.42 Da        Isoelectric Point: 5.1890

>NTDB_id=322732 D9Y32_RS14420 WP_003183693.1 2803701..2804330(+) (comEA) [Bacillus licheniformis strain TCCC 11148]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTYDKKEEVKREKSAGKEAVIIDLKGAVKNPGV
YQMKEGDRVHDVLKKAGGTEKKADQKQINLAAVLQDGMVVYIPFEGEEAADSFSKAGSRADGASVDIVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFHTVEDITNVSGIGEKSFERIKSAITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=322732 D9Y32_RS14420 WP_003183693.1 2803701..2804330(+) (comEA) [Bacillus licheniformis strain TCCC 11148]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCTGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCCGAGGAGGCTTCTGCACAGACGTATGACAAGAAGG
AGGAGGTGAAACGGGAAAAAAGCGCGGGAAAAGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAAGAGGGAGACAGGGTCCACGATGTATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGATCAAAAGCA
AATTAACCTTGCAGCCGTTTTGCAGGACGGTATGGTGGTTTACATTCCATTTGAGGGGGAAGAGGCTGCTGATTCTTTCT
CGAAAGCGGGTTCAAGGGCAGATGGCGCTTCCGTCGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCCGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGCGAGGAGAACGGACCGTTTCACACGGTAGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTGCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(147-207)

SLBB domain (SLBB)

(69-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526

Multiple sequence alignment