Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EBB60_RS00425 Genome accession   NZ_CP033213
Coordinates   50739..52112 (+) Length   457 a.a.
NCBI ID   WP_003421247.1    Uniprot ID   A0AAX3H3G8
Organism   Clostridioides difficile strain CD10010     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 45739..57112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBB60_RS00405 - 46119..46583 (+) 465 WP_003421256.1 CtsR family transcriptional regulator -
  EBB60_RS00410 - 46590..47084 (+) 495 WP_003421255.1 UvrB/UvrC motif-containing protein -
  EBB60_RS00415 - 47100..48125 (+) 1026 WP_003421251.1 protein arginine kinase -
  EBB60_RS00420 - 48112..50559 (+) 2448 WP_003421249.1 ATP-dependent Clp protease ATP-binding subunit -
  EBB60_RS00425 radA 50739..52112 (+) 1374 WP_003421247.1 DNA repair protein RadA Machinery gene
  EBB60_RS00430 disA 52115..53185 (+) 1071 WP_003421245.1 DNA integrity scanning diadenylate cyclase DisA -
  EBB60_RS00435 - 53274..54368 (+) 1095 WP_003421243.1 PIN/TRAM domain-containing protein -
  EBB60_RS00440 - 54497..56674 (+) 2178 WP_003421241.1 hypothetical protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50300.17 Da        Isoelectric Point: 7.5943

>NTDB_id=322641 EBB60_RS00425 WP_003421247.1 50739..52112(+) (radA) [Clostridioides difficile strain CD10010]
MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKKEVFIIDKSSSKPVTINSIESKEEERFTTDINELDRV
LGGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
EVVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKEGSLAGPKLLEHMVDTVLYFEGE
RYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIP
KRTSTGVDYNRVGMLLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTGEVGLTGEV
RAVSFIEKRIAECKKLGFKKIVVPRSNYDVIKDTKGIEIWPVDNLRQAINIVLGRNQ

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=322641 EBB60_RS00425 WP_003421247.1 50739..52112(+) (radA) [Clostridioides difficile strain CD10010]
ATGGCAAAAATAAAAACAAAATATGTATGTCAATCTTGTGGATATGAAACAGCTAAATGGCTTGGTAAATGTCCAGAATG
TACAAAATGGAATACATTCGTAGAAGAAATAGACCAAAAAAGTACCAAAAAAGAGGTCTTTATAATAGATAAATCTTCGT
CTAAACCAGTAACTATAAACTCAATAGAAAGTAAAGAAGAAGAGAGGTTTACAACAGATATAAATGAATTAGATAGAGTG
CTTGGGGGAGGTATAGTTAAAGGTTCTTTAGTACTTGTTGGTGGAGACCCTGGTATAGGTAAATCAACACTTTTAATCCA
AGTATCTAGCAATGTTGCTAATTTAGGAAAAACTGTTTTATATATAACTGGAGAAGAATCAGAATCTCAAATAAAAATGA
GAGCTAAAAGATTAGGTATTAACTCAGAAAATTTATATATATTTGCTGAAAATAATTTAAGTATAATTGAATCTTATTTA
GAAGTTGTAAATCCAGAATTAATAATTCTAGACTCAATTCAAACTGTCTTCAGTCCAGAAATATCCTCGGCACCAGGAAC
TGTCAGCCAAATTAAAGAAGGTACTTCAAAATTCATGAAAATTTCTAAAAAAATGGGAATTTCAACATTCATAGTTGGGC
ATGTTACAAAAGAAGGTTCACTAGCAGGACCAAAATTATTAGAGCATATGGTTGATACTGTTCTATATTTTGAAGGAGAA
AGATATAATACTTATAGATTAGTAAGAGCTGTTAAAAATCGATTTGGCTCAACTAATGAACTTGGAGTATTTGAAATGAG
GGATTTAGGATTAGTGGAACTTGATAATCCGTCTAAAATTTTAATCTCTGAAAAACCAAAAGATGTAGCAGGTTCAGTAA
TAATATCTACTGTAGAAGGTACGAGACCAATGTTACTTGAATTACAAGCATTAGTTTCTCCTACAAGTTTTGGTATACCA
AAAAGAACATCTACAGGAGTCGATTACAATAGAGTAGGTATGTTATTAGCAGTATTAGAAAAACGTGTTGGTTTGCAAAT
ACAAAATCAAGATGTCTATATAAACATAGTTGGAGGAATAAAGATAAATGAACCTTCAATTGACTTAGGGATAGCTATTT
CTGTAGCATCTAGTTTTAGGAATATACCAATAGATGAAGATATAGCTGTCACAGGAGAAGTGGGTCTTACAGGAGAAGTT
AGAGCAGTAAGCTTTATAGAAAAGAGAATAGCTGAGTGTAAAAAACTTGGATTTAAAAAGATAGTAGTTCCTAGAAGTAA
TTATGATGTTATAAAAGATACCAAAGGCATAGAAATATGGCCAGTAGATAACTTAAGACAAGCTATAAATATAGTGCTTG
GGAGGAATCAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(342-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

57.112

100

0.571
  radA Streptococcus mitis NCTC 12261

51.322

99.344

0.51
  radA Streptococcus pneumoniae Rx1

51.322

99.344

0.51
  radA Streptococcus pneumoniae D39

51.322

99.344

0.51
  radA Streptococcus pneumoniae R6

51.322

99.344

0.51
  radA Streptococcus pneumoniae TIGR4

51.322

99.344

0.51
  radA Streptococcus mitis SK321

51.101

99.344

0.508

Multiple sequence alignment