Detailed information NTG0322594

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: DA787_RS19360
• Genome accession: NZ_CP033205
• Coordinates: 3706612..3707757 (+)
• Length: 381 a.a.
• NCBI ID: WP_003227549.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain MBI 600
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3701612..3712757

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DA787_RS19335 (DA787_19320)	albF	3701628..3702908 (+)	1281	WP_003242974.1	pitrilysin family protein	-
DA787_RS19340 (DA787_19325)	albG	3702905..3703606 (+)	702	WP_003243391.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
DA787_RS19345 (DA787_19330)	ywhL	3703612..3704988 (-)	1377	WP_003244415.1	YncE family protein	-
DA787_RS19350 (DA787_19335)	ywhK	3705027..3706382 (-)	1356	WP_003227552.1	YncE family protein	-
DA787_RS21555 (DA787_19340)	-	3706379..3706609 (-)	231	WP_009968328.1	hypothetical protein	-
DA787_RS19360 (DA787_19345)	rapF	3706612..3707757 (+)	1146	WP_003227549.1	response regulator aspartate phosphatase RapF	Regulator
DA787_RS19365 (DA787_19350)	phrF	3707741..3707860 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
DA787_RS19370 (DA787_19355)	ywhH	3707959..3708432 (+)	474	WP_003227547.1	YbaK/EbsC family protein	-
DA787_RS19375 (DA787_19360)	speB	3708464..3709336 (-)	873	WP_003227545.1	agmatinase	-
DA787_RS19380 (DA787_19365)	speE	3709397..3710227 (-)	831	WP_003227543.1	spermidine synthase	-
DA787_RS19385 (DA787_19370)	pbpG	3710429..3712504 (+)	2076	WP_003227540.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45609.09 Da
• Isoelectric Point: 5.1503

>NTDB_id=322594 DA787_RS19360 WP_003227549.1 3706612..3707757(+) (rapF) [Bacillus subtilis strain MBI 600]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=322594 DA787_RS19360 WP_003227549.1 3706612..3707757(+) (rapF) [Bacillus subtilis strain MBI 600]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	100	100	1
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572