Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   D2B33_RS07030 Genome accession   NZ_CP033198
Coordinates   1333757..1334791 (+) Length   344 a.a.
NCBI ID   WP_041817253.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain FA6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1328757..1339791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2B33_RS06990 (D2B33_07030) - 1329253..1329567 (+) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -
  D2B33_RS06995 (D2B33_07035) - 1329628..1329801 (-) 174 WP_003183486.1 DUF2759 domain-containing protein -
  D2B33_RS07000 (D2B33_07040) - 1329957..1330595 (+) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  D2B33_RS07005 (D2B33_07045) - 1330631..1330876 (-) 246 WP_020452180.1 DUF2626 domain-containing protein -
  D2B33_RS07010 (D2B33_07050) - 1331087..1331464 (+) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  D2B33_RS07020 (D2B33_07060) - 1331706..1332545 (+) 840 WP_020452179.1 STAS domain-containing protein -
  D2B33_RS07025 (D2B33_07065) comGA 1332703..1333767 (+) 1065 WP_020452178.1 competence type IV pilus ATPase ComGA Machinery gene
  D2B33_RS07030 (D2B33_07070) comGB 1333757..1334791 (+) 1035 WP_041817253.1 competence type IV pilus assembly protein ComGB Machinery gene
  D2B33_RS07035 (D2B33_07075) comGC 1334805..1335098 (+) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  D2B33_RS07040 (D2B33_07080) comGD 1335104..1335541 (+) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  D2B33_RS07045 (D2B33_07085) comGE 1335525..1335872 (+) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  D2B33_RS07050 (D2B33_07090) comGF 1335787..1336269 (+) 483 WP_228119627.1 competence type IV pilus minor pilin ComGF -
  D2B33_RS07055 (D2B33_07095) comGG 1336281..1336646 (+) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  D2B33_RS07060 (D2B33_07100) - 1336735..1336917 (+) 183 WP_020452171.1 YqzE family protein -
  D2B33_RS07065 (D2B33_07105) - 1336947..1337267 (-) 321 WP_020452170.1 YqzG/YhdC family protein -
  D2B33_RS07070 (D2B33_07110) tapA 1337545..1338273 (+) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  D2B33_RS07075 (D2B33_07115) sipW 1338270..1338854 (+) 585 WP_020452168.1 signal peptidase I SipW -
  D2B33_RS07080 (D2B33_07120) tasA 1338927..1339721 (+) 795 WP_020452167.1 biofilm matrix protein TasA -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39780.12 Da        Isoelectric Point: 9.7341

>NTDB_id=322436 D2B33_RS07030 WP_041817253.1 1333757..1334791(+) (comGB) [Bacillus paralicheniformis strain FA6]
MKIKKKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LSFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGLLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=322436 D2B33_RS07030 WP_041817253.1 1333757..1334791(+) (comGB) [Bacillus paralicheniformis strain FA6]
ATGAAGATTAAAAAGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTATCATTTCATAAAAATGAAGCGAAGCGTCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACTCTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538

Multiple sequence alignment