Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   FGA23_RS27045 Genome accession   NZ_CP040570
Coordinates   5323648..5325477 (-) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli O157:H7 strain ECP17-1298     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5318648..5330477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGA23_RS27020 (FGA23_27020) bioP 5319366..5320265 (-) 900 WP_001196238.1 biotin transporter -
  FGA23_RS27025 (FGA23_27025) yigL 5320341..5321141 (-) 801 WP_000285328.1 sugar/pyridoxal phosphate phosphatase YigL -
  FGA23_RS27030 (FGA23_27030) pldB 5321149..5322171 (-) 1023 WP_000487652.1 lysophospholipase L2 -
  FGA23_RS27035 (FGA23_27035) rhtB 5322282..5322902 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  FGA23_RS27040 (FGA23_27040) rhtC 5322964..5323584 (-) 621 WP_000928824.1 threonine export protein RhtC -
  FGA23_RS27045 (FGA23_27045) recQ 5323648..5325477 (-) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  FGA23_RS27050 (FGA23_27050) pldA 5325610..5326479 (-) 870 WP_001259700.1 phospholipase A -
  FGA23_RS27055 (FGA23_27055) yigI 5326644..5327111 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  FGA23_RS27060 (FGA23_27060) rarD 5327163..5328050 (+) 888 WP_000339106.1 EamA family transporter RarD -
  FGA23_RS27065 (FGA23_27065) - 5328097..5328396 (-) 300 WP_000526425.1 hypothetical protein -
  FGA23_RS27075 (FGA23_27075) - 5329023..5329169 (-) 147 Protein_5200 CorA family divalent cation transporter -
  FGA23_RS27080 (FGA23_27080) - 5329356..5330204 (+) 849 WP_001066403.1 DUF3289 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=322147 FGA23_RS27045 WP_000035581.1 5323648..5325477(-) (recQ) [Escherichia coli O157:H7 strain ECP17-1298]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=322147 FGA23_RS27045 WP_000035581.1 5323648..5325477(-) (recQ) [Escherichia coli O157:H7 strain ECP17-1298]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCTGCGGAAGCGATGTTGTTTTACGATCCAGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTACAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGTAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGCGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391