Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CDG68_RS18945 Genome accession   NZ_CP033133
Coordinates   3370040..3370843 (-) Length   267 a.a.
NCBI ID   WP_087553575.1    Uniprot ID   A0A3G2T904
Organism   Acinetobacter wuhouensis strain WCHAW010062     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3365040..3375843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG68_RS18925 (CDG68_18930) - 3366021..3366764 (-) 744 WP_068973271.1 YfgM family protein -
  CDG68_RS18930 (CDG68_18935) hisS 3366819..3368114 (-) 1296 WP_087553573.1 histidine--tRNA ligase -
  CDG68_RS18935 (CDG68_18940) ispG 3368118..3369236 (-) 1119 WP_068973269.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CDG68_RS18940 (CDG68_18945) - 3369258..3370049 (-) 792 WP_087553574.1 helix-turn-helix domain-containing protein -
  CDG68_RS18945 (CDG68_18950) pilF 3370040..3370843 (-) 804 WP_087553575.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CDG68_RS18950 (CDG68_18955) rlmN 3370897..3372147 (-) 1251 WP_087553576.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CDG68_RS18955 (CDG68_18960) ndk 3372326..3372757 (-) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  CDG68_RS18960 (CDG68_18965) iscX 3372890..3373090 (-) 201 WP_087553577.1 Fe-S cluster assembly protein IscX -
  CDG68_RS18965 (CDG68_18970) - 3373306..3373857 (-) 552 WP_087553578.1 phosphatase PAP2 family protein -
  CDG68_RS18970 (CDG68_18975) - 3373888..3375174 (-) 1287 WP_087553579.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29949.21 Da        Isoelectric Point: 9.5545

>NTDB_id=321660 CDG68_RS18945 WP_087553575.1 3370040..3370843(-) (pilF) [Acinetobacter wuhouensis strain WCHAW010062]
MYSKAIVMLTIGLSALAIQGCQTTSTGPISKKDPEKAVKVRTQLAAEYIRSGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDPKNAQARNNYGTYLFISERYNDAIEQLSIAGAALGYDQRYKALENLGRVYLKVGD
VVNAEKAFKQALQVNRDLVISLVELSEIFYLQQKNAQARQLYEQYVRLVGQDKQGARALWIGLRIARANQDQMGMQVLVN
QLRALFPDSKEYQRYLQLQNSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=321660 CDG68_RS18945 WP_087553575.1 3370040..3370843(-) (pilF) [Acinetobacter wuhouensis strain WCHAW010062]
ATGTATTCAAAAGCAATTGTAATGTTGACAATTGGTTTATCCGCATTGGCTATTCAAGGTTGTCAGACGACAAGTACTGG
TCCAATAAGTAAAAAAGATCCAGAGAAAGCCGTCAAAGTACGTACCCAACTTGCTGCAGAATACATCCGTTCAGGTGATT
TGGATGCAGCAAAACGTGCATTAGATCAAGCGCTTGAAACCGATTCACGGGATTCAGCTGCCAATATGATGATGGGTGTT
TTACTGCAACAAGAGGGCAGTAAAATCAGTATGGAAAAAGCAGATGGCTATTTTAAGCGTGCAATTTCTAGTGACCCAAA
AAATGCACAAGCTCGTAATAATTATGGAACATATTTATTTATTAGTGAGCGTTATAATGATGCCATCGAACAGTTGAGCA
TAGCTGGTGCTGCATTAGGATATGATCAGCGTTACAAAGCTTTAGAAAATTTGGGTCGCGTGTATTTGAAGGTAGGCGAT
GTGGTCAATGCTGAAAAAGCATTCAAACAAGCCTTGCAAGTGAATCGCGATTTAGTCATTTCTTTGGTAGAGTTATCTGA
GATTTTTTATTTGCAGCAAAAAAATGCACAAGCACGTCAACTCTATGAGCAATATGTTCGTTTAGTAGGTCAGGATAAAC
AAGGTGCACGTGCACTTTGGATTGGATTGCGTATTGCACGTGCCAATCAAGACCAGATGGGAATGCAAGTGTTGGTGAAT
CAACTTCGTGCATTATTCCCTGACAGTAAAGAATATCAACGTTATCTGCAATTGCAGAATAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(44-79)

Tetratricopeptide repeat (TPR_16)

(94-133)

Tetratricopeptide repeat (TPR_16)

(150-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G2T904

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

75.573

98.127

0.742
  pilF Acinetobacter baylyi ADP1

65.799

100

0.663

Multiple sequence alignment