Detailed information NTG0321506

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: FEL12_RS25545
• Genome accession: NZ_CP040316
• Coordinates: 5168548..5169531 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain CV261
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 5163548..5174531

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FEL12_RS25520 (FEL12_26245)	bcsF	5163709..5163900 (+)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
FEL12_RS25525 (FEL12_26250)	bcsG	5163897..5165576 (+)	1680	WP_001691049.1	cellulose biosynthesis protein BcsG	-
FEL12_RS25530 (FEL12_26255)	ldrD	5165663..5165770 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
FEL12_RS25535 (FEL12_26265)	yhjV	5166246..5167517 (+)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
FEL12_RS25540 (FEL12_26270)	dppF	5167547..5168551 (-)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
FEL12_RS25545 (FEL12_26275)	amiE	5168548..5169531 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
FEL12_RS25550 (FEL12_26280)	dppC	5169542..5170444 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
FEL12_RS25555 (FEL12_26285)	dppB	5170454..5171473 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
FEL12_RS25560 (FEL12_26295)	dppA	5171624..5173231 (-)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=321506 FEL12_RS25545 WP_001196486.1 5168548..5169531(-) (amiE) [Escherichia coli strain CV261]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=321506 FEL12_RS25545 WP_001196486.1 5168548..5169531(-) (amiE) [Escherichia coli strain CV261]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404