Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EAE94_RS16050 Genome accession   NZ_CP033108
Coordinates   3343038..3343838 (-) Length   266 a.a.
NCBI ID   WP_179994561.1    Uniprot ID   -
Organism   Acinetobacter baumannii ATCC 17978     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3338038..3348838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAE94_RS16030 (EAE94_16110) - 3339103..3339801 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  EAE94_RS16035 (EAE94_16115) hisS 3339830..3341122 (-) 1293 WP_000095263.1 histidine--tRNA ligase -
  EAE94_RS16040 (EAE94_16120) ispG 3341119..3342234 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EAE94_RS16045 (EAE94_16125) - 3342256..3343047 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  EAE94_RS16050 (EAE94_16130) pilF 3343038..3343838 (-) 801 WP_179994561.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EAE94_RS16055 (EAE94_16135) rlmN 3343854..3345086 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EAE94_RS16060 (EAE94_16140) ndk 3345209..3345640 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  EAE94_RS16065 (EAE94_16145) iscX 3345754..3345954 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  EAE94_RS16070 (EAE94_16150) - 3346054..3346605 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  EAE94_RS16075 (EAE94_16155) - 3346630..3347928 (-) 1299 WP_104159583.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30075.39 Da        Isoelectric Point: 9.0211

>NTDB_id=321460 EAE94_RS16050 WP_179994561.1 3343038..3343838(-) (pilF) [Acinetobacter baumannii ATCC 17978]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=321460 EAE94_RS16050 WP_179994561.1 3343038..3343838(-) (pilF) [Acinetobacter baumannii ATCC 17978]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACATAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment