Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   D9777_RS20560 Genome accession   NZ_CP033054
Coordinates   4087211..4088356 (+) Length   381 a.a.
NCBI ID   WP_014419129.1    Uniprot ID   -
Organism   Bacillus velezensis strain Bac57     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 4082211..4093356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9777_RS20540 - 4082686..4083402 (-) 717 WP_014419126.1 Crp/Fnr family transcriptional regulator -
  D9777_RS20545 narK 4083500..4084690 (-) 1191 WP_007407632.1 nitrate transporter NarK -
  D9777_RS20550 argS 4084824..4086494 (-) 1671 WP_007407633.1 arginine--tRNA ligase -
  D9777_RS20555 - 4086491..4086919 (-) 429 WP_014419128.1 DUF1934 family protein -
  D9777_RS20560 rapF 4087211..4088356 (+) 1146 WP_014419129.1 tetratricopeptide repeat protein Regulator
  D9777_RS20565 - 4088340..4088459 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  D9777_RS20570 - 4088657..4088860 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  D9777_RS20575 - 4088874..4089077 (+) 204 WP_007407641.1 hypothetical protein -
  D9777_RS20580 - 4089112..4089288 (+) 177 Protein_3963 DUF4177 domain-containing protein -
  D9777_RS20585 - 4089483..4089764 (+) 282 WP_007407643.1 hypothetical protein -
  D9777_RS20590 - 4089808..4090320 (+) 513 WP_031304384.1 stage II sporulation protein M -
  D9777_RS20600 - 4090911..4091129 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  D9777_RS20605 speB 4091284..4092156 (-) 873 WP_007614448.1 agmatinase -
  D9777_RS20610 speE 4092215..4093045 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45581.65 Da        Isoelectric Point: 4.5797

>NTDB_id=320972 D9777_RS20560 WP_014419129.1 4087211..4088356(+) (rapF) [Bacillus velezensis strain Bac57]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSELLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNTAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=320972 D9777_RS20560 WP_014419129.1 4087211..4088356(+) (rapF) [Bacillus velezensis strain Bac57]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGAGCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATACGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.215

100

0.782
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment