Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   D9777_RS06790 Genome accession   NZ_CP033054
Coordinates   1312326..1316012 (+) Length   1228 a.a.
NCBI ID   WP_031304443.1    Uniprot ID   -
Organism   Bacillus velezensis strain Bac57     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1307326..1321012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9777_RS06775 - 1307407..1307916 (-) 510 WP_007610501.1 ferritin-like domain-containing protein -
  D9777_RS06780 - 1307985..1308698 (-) 714 WP_017417413.1 DUF421 domain-containing protein -
  D9777_RS06785 addB 1308839..1312339 (+) 3501 WP_021494951.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  D9777_RS06790 addA 1312326..1316012 (+) 3687 WP_031304443.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  D9777_RS06795 sbcD 1316077..1317249 (+) 1173 WP_021494953.1 exonuclease subunit SbcD -
  D9777_RS06800 sbcC 1317246..1320638 (+) 3393 WP_021494954.1 exonuclease subunit SbcC -
  D9777_RS06805 - 1320654..1320947 (+) 294 WP_021494955.1 hypothetical protein -

Sequence

Protein


Download         Length: 1228 a.a.        Molecular weight: 140600.04 Da        Isoelectric Point: 5.2183

>NTDB_id=320920 D9777_RS06790 WP_031304443.1 1312326..1316012(+) (addA) [Bacillus velezensis strain Bac57]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLQSFVRLYDVTEESKMEELPFYQYVREDVEMALFGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKVK
TDYFTRSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSAEDAEDFETVHWEAKAIAGEIRKLVSSP
FKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDIPLASVL
RSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRDTGYFDY
AGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFPVVFT
AGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLAEELRVLYVALTRAKEKLFLVGSCKN
REKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSSEDIARDPSRFHIRMLQQSELLEENP
KERAEEKSKRLKAIQQGEPIPGSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPSGDTLLY
RRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVQFFGTEIGKDLLGAL
RIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYETQIELY
TKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3687 bp        

>NTDB_id=320920 D9777_RS06790 WP_031304443.1 1312326..1316012(+) (addA) [Bacillus velezensis strain Bac57]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAAGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGTCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCGGACAGATATACGACAGACAGGCATGATCTGGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGCAAAGCTTTGTCCGCTTGTATGACGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAGTATGTGAGAGAAGATGTTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTTGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCATTTAAGCGGGCGAAGGCGGTAAAGG
GTGATGAATTTGACAAAGCGCTTTTGGACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAG
ACAGATTACTTTACAAGAAGTCCGCAAGATCACTTAAAGAGTTTAACAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGGTTGATGAAGAAGGCCGGCGGGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTTCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGATCTGAACCAAAATTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCGTATCCGCCGAATGACGCGGCCAAAACGGAACTGCTGCTGATTGACAGCGCCG
AGGATGCCGAAGACTTTGAAACCGTGCACTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGAAAGCTTGTCTCATCTCCG
TTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGATTTTGCTGCGTTCCATGCC
GTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGACGTCGGGTTACTTTGAAG
CCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATTCCGCTTGCTTCCGTGCTG
CGCTCGCCCATCGTCGGATGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAAAGCGCCGTTTTATGAAGC
GCTGAAAGCATATATAGCGAACGGTGACAGGCATGATGACCTGTATCAAAAACTGAGAACCTTTTATGACAGTCTGCAAA
AGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGATACCGGATATTTTGACTAT
GCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGCGCGCTCGTACGAAGCGAC
GGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATGATCTGGGGACGGCCCGGG
CGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAATTTCCCGTCGTTTTCACC
GCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGAGCTCGGTTTCGGCACGAA
GTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTGCCGCTTGTGGCGATGAAGAAAAAGATGCGCAGAGAGCTCT
TAGCGGAAGAATTGCGCGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTCGTCGGTTCCTGCAAAAAC
CGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCTGATTGGCTTCTTTCTGAATTCGACCGCTATCAGGC
GTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACAGGACGCCGGGACTTTCAT
CGTCTGAGGATATCGCGCGCGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAATTGCTGGAAGAGAATCCA
AAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAAGCGATTCAGCAGGGCGAGCCGATTCCTGGTTCGTTTTCATTTGA
TGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAACGAAGCAGTCAGTTTCTG
AGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCAGGAGACACGCTTTTATAC
AGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCTGCCGAAAAAGGAACGGCGATGCATACGGTGATGCAGCACAT
ACCGCTGACGCACGTGCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAAAAGAGCTGCTGACAGAAG
AGCAGCAGGAAGCTATTGATATAGAAGAAATTGTTCAGTTTTTCGGAACTGAAATCGGTAAAGATCTTCTCGGGGCGCTG
CGGATTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGCCGAAACGGCCGGTGAACC
GCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACGGCGGACGGTCTCTATTTGCTGGACTATAAAACAGATCGGA
TAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGGTATGAAACACAAATCGAATTGTAT
ACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGATGGAGGTCACGTTCTTAC
ACTGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1049-1220)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-875)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.496

100

0.829

Multiple sequence alignment