Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   D9M09_RS00510 Genome accession   NZ_CP033019
Coordinates   98622..100100 (+) Length   492 a.a.
NCBI ID   WP_070221651.1    Uniprot ID   A0A853SV47
Organism   Janthinobacterium agaricidamnosum strain BHSEK     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93622..105100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9M09_RS00485 (D9M09_00485) - 95058..96068 (+) 1011 WP_083287308.1 KpsF/GutQ family sugar-phosphate isomerase -
  D9M09_RS00490 (D9M09_00490) - 96068..96601 (+) 534 WP_121668346.1 HAD hydrolase family protein -
  D9M09_RS00495 (D9M09_00495) lptC 96644..97255 (+) 612 WP_070221653.1 LPS export ABC transporter periplasmic protein LptC -
  D9M09_RS00500 (D9M09_00500) lptA 97281..97862 (+) 582 WP_121668347.1 lipopolysaccharide transport periplasmic protein LptA -
  D9M09_RS00505 (D9M09_00505) lptB 97862..98617 (+) 756 WP_034753325.1 LPS export ABC transporter ATP-binding protein -
  D9M09_RS00510 (D9M09_00510) rpoN/rpoN1 98622..100100 (+) 1479 WP_070221651.1 RNA polymerase factor sigma-54 Machinery gene
  D9M09_RS00515 (D9M09_00515) raiA 100170..100529 (+) 360 WP_070221650.1 ribosome-associated translation inhibitor RaiA -
  D9M09_RS00520 (D9M09_00520) - 100661..101746 (+) 1086 WP_121668348.1 enoyl-CoA hydratase/isomerase family protein -
  D9M09_RS00525 (D9M09_00525) rapZ 101747..102610 (-) 864 WP_070221648.1 RNase adapter RapZ -
  D9M09_RS00535 (D9M09_00535) - 103006..103965 (+) 960 WP_121668350.1 IS110 family transposase -
  D9M09_RS00540 (D9M09_00540) hprK 103989..104924 (-) 936 WP_034753337.1 HPr(Ser) kinase/phosphatase -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54537.23 Da        Isoelectric Point: 5.1075

>NTDB_id=320486 D9M09_RS00510 WP_070221651.1 98622..100100(+) (rpoN/rpoN1) [Janthinobacterium agaricidamnosum strain BHSEK]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSLRLLSDGALSSTAAPAEAPPQPP
GQEAPAAPAEAETFDGEAGEGPAAADGGDSDWSEASRGKAPDDEDSRPQLEAHHCTLREHLMEQMRVTVLELRDRALVEL
IIDALDDNGYLEESLDDILARLPEELEIDADEMRTALSLLQSFDPPGVGARNASECLALQIKRLPHIAMVTRRMALVIVE
KHLAWFAQRDFNKLKKALDCDDEDLREAQTVIRQCNPHPGAVFAADVSDYVVPDVVVKRARNGWQVTLNNDVMPRLRVNA
MYANLLKQGKGEGAMGAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRV
TTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREA
LKIPPVSLRKCL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=320486 D9M09_RS00510 WP_070221651.1 98622..100100(+) (rpoN/rpoN1) [Janthinobacterium agaricidamnosum strain BHSEK]
ATGAAACAATCATTGCAGCTGCGCACTTCGCAGCACCTGGCACTGACGCCGCAGTTGCAGCAATCGATACGCCTGTTGCA
ATTGTCTACTCTGGAATTGCACCAGGAACTCGAGCAACTGCTGACGGACAATCCCTTGCTCGAGCGCCTCGACGATCCGC
TCGACCGTTCGCTGCGCCTGCTGTCCGACGGGGCCTTGAGCTCCACGGCCGCGCCGGCCGAAGCGCCGCCCCAGCCGCCA
GGCCAGGAGGCGCCCGCGGCGCCGGCCGAAGCGGAAACCTTTGACGGCGAGGCGGGCGAGGGCCCGGCCGCCGCGGATGG
CGGCGACAGCGACTGGAGCGAGGCGAGCCGGGGCAAGGCGCCCGACGACGAAGATTCCCGCCCGCAACTGGAGGCCCATC
ACTGCACCCTGCGCGAACACCTGATGGAGCAGATGCGCGTGACGGTGCTCGAATTGCGCGACCGCGCGCTGGTCGAGCTG
ATCATCGACGCGCTCGACGACAATGGCTACCTGGAAGAGTCGCTCGACGACATCCTGGCGCGCCTGCCGGAAGAGCTGGA
AATCGATGCCGACGAGATGCGCACGGCCTTGTCGCTGCTGCAAAGTTTCGATCCACCGGGCGTGGGCGCGCGCAATGCGT
CCGAATGCCTGGCGCTGCAAATCAAGCGCTTGCCGCACATCGCCATGGTGACGCGGCGCATGGCCCTCGTCATCGTGGAA
AAGCACCTGGCCTGGTTTGCCCAGCGCGATTTTAACAAGCTGAAAAAAGCCCTCGATTGCGACGATGAAGACTTGCGTGA
AGCGCAGACGGTAATTCGCCAGTGCAACCCGCATCCGGGCGCCGTGTTCGCTGCGGACGTGTCCGATTACGTGGTGCCCG
ACGTCGTCGTCAAGCGCGCGCGCAATGGCTGGCAAGTGACCCTGAACAACGACGTCATGCCGCGCCTGCGCGTCAACGCC
ATGTACGCCAACTTGCTCAAGCAGGGCAAGGGCGAGGGCGCCATGGGTGCACAGTTGCAGGAAGCCAAGTGGCTGATCAA
GAATATGCGCCAGCGCTTCGACACGATTCTGCGCGTGGCGCAGGCGATAGTAGAAAGACAAAAGAACTTTTTCTCGCATG
GGGCCGTTGCCATGCGCCCCCTTGTGCTCCGTGAAATAGCTGATACACTGGGTTTACACGAGAGTACTATCTCGCGGGTA
ACAACTCAAAAGTACATGCTGACCCCGCACGGCATGTTCGAGTTGAAATATTTCTTTGGTAGCCACGTCGCCACCGAAGC
GGGGGGCGAAGCGTCATCGACGGCGATACGGGCATTGATCGTGCAACTGACAGGAGCAGAAGACCCTAAAAATCCTTTAT
CCGACAGTAAGATTGCGGACATGCTGGGGGAACAAGGCATGGTGATTGCGCGACGTACTGTTGCCAAATACCGGGAAGCG
TTAAAAATTCCGCCAGTGAGCCTGCGTAAGTGTTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-322)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-490)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A853SV47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.714

100

0.622

Multiple sequence alignment