Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   D9C08_RS00335 Genome accession   NZ_CP032872
Coordinates   65420..66811 (-) Length   463 a.a.
NCBI ID   WP_014481052.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain 2KL1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 60420..71811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C08_RS00300 (D9C08_00300) flgL 60592..61488 (-) 897 WP_014481046.1 flagellar hook-associated protein FlgL -
  D9C08_RS00305 (D9C08_00305) flgK 61499..63022 (-) 1524 WP_014481047.1 flagellar hook-associated protein FlgK -
  D9C08_RS00310 (D9C08_00310) flgN 63041..63523 (-) 483 WP_014481048.1 flagellar protein FlgN -
  D9C08_RS00315 (D9C08_00315) flgM 63539..63805 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  D9C08_RS00320 (D9C08_00320) yvyF 63886..64305 (-) 420 WP_038429728.1 TIGR03826 family flagellar region protein -
  D9C08_RS00325 (D9C08_00325) comFC 64378..65100 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  D9C08_RS00330 (D9C08_00330) comFB 65064..65360 (-) 297 WP_003227989.1 late competence protein ComFB -
  D9C08_RS00335 (D9C08_00335) comFA 65420..66811 (-) 1392 WP_014481052.1 ATP-dependent helicase ComFA Machinery gene
  D9C08_RS00340 (D9C08_00340) fakBA 66918..67763 (-) 846 WP_014481053.1 DegV family protein -
  D9C08_RS00345 (D9C08_00345) degU 67861..68550 (-) 690 WP_014481054.1 two-component system response regulator DegU Regulator
  D9C08_RS00350 (D9C08_00350) degS 68633..69790 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  D9C08_RS00355 (D9C08_00355) - 70007..70660 (+) 654 WP_014481055.1 YigZ family protein -
  D9C08_RS00360 (D9C08_00360) - 70660..71652 (+) 993 WP_014481056.1 LCP family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52543.49 Da        Isoelectric Point: 9.7976

>NTDB_id=319885 D9C08_RS00335 WP_014481052.1 65420..66811(-) (comFA) [Bacillus subtilis subsp. subtilis strain 2KL1]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=319885 D9C08_RS00335 WP_014481052.1 65420..66811(-) (comFA) [Bacillus subtilis subsp. subtilis strain 2KL1]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.056

100

0.981

Multiple sequence alignment