Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   D9C20_RS06700 Genome accession   NZ_CP032867
Coordinates   1244981..1246372 (-) Length   463 a.a.
NCBI ID   WP_014481052.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain N4-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1239981..1251372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C20_RS06665 (D9C20_06665) flgL 1240153..1241049 (-) 897 WP_014481046.1 flagellar hook-associated protein FlgL -
  D9C20_RS06670 (D9C20_06670) flgK 1241060..1242583 (-) 1524 WP_014481047.1 flagellar hook-associated protein FlgK -
  D9C20_RS06675 (D9C20_06675) flgN 1242602..1243084 (-) 483 WP_014481048.1 flagellar protein FlgN -
  D9C20_RS06680 (D9C20_06680) flgM 1243100..1243366 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  D9C20_RS06685 (D9C20_06685) yvyF 1243447..1243866 (-) 420 WP_014481050.1 TIGR03826 family flagellar region protein -
  D9C20_RS06690 (D9C20_06690) comFC 1243939..1244661 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  D9C20_RS06695 (D9C20_06695) comFB 1244625..1244921 (-) 297 WP_003227989.1 late competence protein ComFB -
  D9C20_RS06700 (D9C20_06700) comFA 1244981..1246372 (-) 1392 WP_014481052.1 ATP-dependent helicase ComFA Machinery gene
  D9C20_RS06705 (D9C20_06705) fakBA 1246479..1247324 (-) 846 WP_014481053.1 DegV family protein -
  D9C20_RS06710 (D9C20_06710) degU 1247422..1248111 (-) 690 WP_014481054.1 two-component system response regulator DegU Regulator
  D9C20_RS06715 (D9C20_06715) degS 1248194..1249351 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  D9C20_RS06720 (D9C20_06720) - 1249568..1250221 (+) 654 WP_014481055.1 YigZ family protein -
  D9C20_RS06725 (D9C20_06725) - 1250221..1251213 (+) 993 WP_014481056.1 LCP family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52543.49 Da        Isoelectric Point: 9.7976

>NTDB_id=319797 D9C20_RS06700 WP_014481052.1 1244981..1246372(-) (comFA) [Bacillus subtilis subsp. subtilis strain N4-2]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=319797 D9C20_RS06700 WP_014481052.1 1244981..1246372(-) (comFA) [Bacillus subtilis subsp. subtilis strain N4-2]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.056

100

0.981

Multiple sequence alignment