Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D9C14_RS21995 Genome accession   NZ_CP032860
Coordinates   4175204..4175614 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain SSJ-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4170204..4180614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C14_RS21965 (D9C14_21965) - 4170326..4170466 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  D9C14_RS21970 (D9C14_21970) yqeF 4170772..4171503 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  D9C14_RS21975 (D9C14_21975) cwlH 4171755..4172507 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  D9C14_RS21980 (D9C14_21980) yqeD 4172694..4173320 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  D9C14_RS21985 (D9C14_21985) gnd 4173339..4174207 (-) 869 Protein_4262 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  D9C14_RS21990 (D9C14_21990) yqeB 4174449..4175171 (+) 723 WP_010886572.1 hypothetical protein -
  D9C14_RS21995 (D9C14_21995) nucA/comI 4175204..4175614 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  D9C14_RS22000 (D9C14_22000) sigK 4175810..4176538 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  D9C14_RS22005 (D9C14_22005) - 4176538..4176654 (+) 117 WP_003229898.1 hypothetical protein -
  D9C14_RS22010 (D9C14_22010) - 4176620..4176820 (-) 201 Protein_4267 recombinase family protein -
  D9C14_RS22015 (D9C14_22015) - 4176763..4176927 (-) 165 WP_010886576.1 hypothetical protein -
  D9C14_RS22020 (D9C14_22020) psiE 4177472..4177888 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  D9C14_RS22025 (D9C14_22025) yrkQ 4177934..4179232 (-) 1299 WP_019712554.1 two-component system sensor histidine kinase YrkQ -
  D9C14_RS22030 (D9C14_22030) yrkP 4179219..4179912 (-) 694 Protein_4271 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=319529 D9C14_RS21995 WP_009967785.1 4175204..4175614(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SSJ-1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=319529 D9C14_RS21995 WP_009967785.1 4175204..4175614(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SSJ-1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment