Detailed information NTG0031952

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: THA_RS09510
• Genome accession: NC_011653
• Coordinates: 1890191..1891075 (+)
• Length: 294 a.a.
• NCBI ID: WP_004102891.1
• Uniprot ID: B7IE87
• Organism: Thermosipho africanus TCF52B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1885191..1896075

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THA_RS09475 (THA_1881)	-	1885370..1886293 (+)	924	WP_004102880.1	PEGA domain-containing protein	-
THA_RS09480 (THA_1882)	prfB	1886308..1887417 (+)	1110	WP_004102881.1	peptide chain release factor 2	-
THA_RS09485 (THA_1883)	minD	1887429..1888232 (+)	804	WP_004102882.1	septum site-determining protein MinD	-
THA_RS09490 (THA_1884)	-	1888238..1888504 (+)	267	WP_004102883.1	hypothetical protein	-
THA_RS09495 (THA_1885)	-	1888531..1889154 (+)	624	WP_004102886.1	hypothetical protein	-
THA_RS09500 (THA_1886)	-	1889171..1889407 (+)	237	WP_373419040.1	hypothetical protein	-
THA_RS09505 (THA_1887)	-	1889404..1890186 (+)	783	WP_012580469.1	class I SAM-dependent methyltransferase	-
THA_RS09510 (THA_1888)	pilA	1890191..1891075 (+)	885	WP_004102891.1	signal recognition particle-docking protein FtsY	Machinery gene
THA_RS09515 (THA_1889)	-	1891081..1891746 (+)	666	WP_004102893.1	DUF2225 domain-containing protein	-
THA_RS09520 (THA_1890)	-	1891725..1893320 (+)	1596	WP_004102895.1	3D domain-containing protein	-
THA_RS09525 (THA_1891)	-	1893324..1893770 (+)	447	WP_004102897.1	Rrf2 family transcriptional regulator	-
THA_RS09530 (THA_1892)	mnmA	1893767..1894825 (+)	1059	WP_081429717.1	tRNA 2-thiouridine(34) synthase MnmA	-
THA_RS09535 (THA_1893)	hpt	1894822..1895334 (+)	513	WP_004102901.1	hypoxanthine phosphoribosyltransferase	-

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32242.28 Da
• Isoelectric Point: 5.5457

>NTDB_id=31952 THA_RS09510 WP_004102891.1 1890191..1891075(+) (pilA) [Thermosipho africanus TCF52B]
MGFFEKFKQGLKKTRESFFGKMKSILSGKKLDDETKEEIEELLILSDVGFEATEYILNRIEELKGEDAYESLKKVLLEIL
NFNSELKIEKKPFVISMVGVNGSGKTTTAGKLAEYFSKSGKSVVLAACDTFRAAAIDQLKVWAERTSSTFIAHQEGADSA
AVAFDAVSHAISKEKDIVILDTAGRLHTKKNLMEELRKINRVIKKKIEDAPHEVLLVIDAVTGQNGLQQAKIFKEFVDIT
GIVLTKLDGTAKGGIAIAIAKELGIPIKFVGLGEGVDDLKPFDAEDFVNALLEG

Nucleotide

• Length: 885 bp

>NTDB_id=31952 THA_RS09510 WP_004102891.1 1890191..1891075(+) (pilA) [Thermosipho africanus TCF52B]
ATGGGATTTTTCGAAAAGTTCAAGCAAGGATTGAAAAAAACGAGAGAAAGTTTTTTTGGTAAAATGAAAAGTATCCTATC
AGGAAAAAAACTCGATGATGAAACAAAAGAAGAGATAGAAGAGCTTTTAATATTATCTGATGTAGGCTTTGAAGCAACTG
AGTATATTTTAAATAGAATTGAGGAGTTAAAGGGAGAAGATGCATATGAATCGTTGAAAAAAGTATTATTGGAAATACTT
AATTTTAATAGTGAATTAAAAATAGAAAAAAAACCTTTTGTAATTAGTATGGTTGGTGTTAATGGTTCGGGAAAAACTAC
CACTGCTGGAAAACTTGCAGAATATTTTTCAAAGTCTGGAAAGTCAGTTGTGTTAGCTGCATGTGATACTTTTAGAGCTG
CTGCAATTGATCAACTAAAGGTATGGGCGGAGAGAACATCTTCTACGTTTATTGCGCATCAAGAGGGTGCAGATTCTGCT
GCCGTTGCATTTGATGCTGTAAGTCATGCTATATCTAAAGAGAAAGATATAGTAATTTTAGATACTGCTGGAAGATTACA
TACCAAGAAAAATTTAATGGAAGAATTAAGAAAAATTAATAGGGTAATTAAGAAGAAAATCGAAGATGCGCCACACGAAG
TTTTATTAGTAATAGATGCTGTAACAGGTCAAAATGGTTTGCAACAGGCAAAGATCTTTAAAGAATTTGTAGATATTACA
GGTATTGTATTGACAAAGTTGGATGGAACAGCAAAAGGTGGAATTGCCATTGCAATTGCAAAGGAATTAGGGATTCCTAT
AAAATTTGTAGGTTTAGGGGAAGGCGTTGACGATCTAAAACCGTTTGATGCTGAAGATTTTGTTAATGCTTTACTTGAGG
GATGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (20-74)

• SRP54-type protein, GTPase domain (SRP54) (92-292)

3D structure

• AlphaFold DB: B7IE87

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	46.865	100	0.483