Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D9C14_RS10135 Genome accession   NZ_CP032860
Coordinates   1926569..1927018 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis subsp. subtilis strain SSJ-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1921569..1932018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C14_RS10100 (D9C14_10100) yckA 1921720..1922400 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  D9C14_RS10105 (D9C14_10105) yckB 1922410..1923273 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  D9C14_RS10110 (D9C14_10110) - 1923435..1923632 (+) 198 WP_119122841.1 hypothetical protein -
  D9C14_RS10115 (D9C14_10115) yckC 1923651..1924106 (+) 456 WP_003246356.1 RDD family protein -
  D9C14_RS10120 (D9C14_10120) yckD 1924188..1924520 (+) 333 WP_003246247.1 YckD family protein -
  D9C14_RS10125 (D9C14_10125) bglC 1924674..1926107 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  D9C14_RS10130 (D9C14_10130) nin/comJ 1926144..1926542 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  D9C14_RS10135 (D9C14_10135) nucA/comI 1926569..1927018 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  D9C14_RS10140 (D9C14_10140) tlpC 1927186..1928907 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  D9C14_RS10145 (D9C14_10145) hxlB 1929018..1929575 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  D9C14_RS10150 (D9C14_10150) hxlA 1929581..1930213 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  D9C14_RS10155 (D9C14_10155) hxlR 1930446..1930808 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=319484 D9C14_RS10135 WP_003246436.1 1926569..1927018(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SSJ-1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=319484 D9C14_RS10135 WP_003246436.1 1926569..1927018(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SSJ-1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment