Detailed information NTG0319449

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: D9C12_RS21800
• Genome accession: NZ_CP032857
• Coordinates: 4062324..4063469 (+)
• Length: 381 a.a.
• NCBI ID: WP_069837832.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain 2RL2-3
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 4057324..4068469

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9C12_RS21775 (D9C12_21775)	albF	4057344..4058624 (+)	1281	WP_069837829.1	pitrilysin family protein	-
D9C12_RS21780 (D9C12_21780)	albG	4058621..4059322 (+)	702	WP_069837830.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
D9C12_RS21785 (D9C12_21785)	ywhL	4059328..4060704 (-)	1377	WP_069837831.1	YncE family protein	-
D9C12_RS21790 (D9C12_21790)	ywhK	4060743..4062094 (-)	1352	Protein_4211	YncE family protein	-
D9C12_RS24125 (D9C12_21795)	-	4062091..4062321 (-)	231	Protein_4212	hypothetical protein	-
D9C12_RS21800 (D9C12_21800)	rapF	4062324..4063469 (+)	1146	WP_069837832.1	response regulator aspartate phosphatase RapF	Regulator
D9C12_RS21805 (D9C12_21805)	phrF	4063453..4063572 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
D9C12_RS21810 (D9C12_21810)	-	4063670..4064097 (+)	428	Protein_4215	YbaK/EbsC family protein	-
D9C12_RS21815 (D9C12_21815)	speB	4064162..4065034 (-)	873	WP_003227545.1	agmatinase	-
D9C12_RS21820 (D9C12_21820)	speE	4065095..4065925 (-)	831	WP_003227543.1	spermidine synthase	-
D9C12_RS21825 (D9C12_21825)	pbpG	4066127..4068202 (+)	2076	WP_069837833.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45624.07 Da
• Isoelectric Point: 5.0624

>NTDB_id=319449 D9C12_RS21800 WP_069837832.1 4062324..4063469(+) (rapF) [Bacillus subtilis subsp. subtilis strain 2RL2-3]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFTIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=319449 D9C12_RS21800 WP_069837832.1 4062324..4063469(+) (rapF) [Bacillus subtilis subsp. subtilis strain 2RL2-3]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACAGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCGTCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACATATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCACTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	98.688	100	0.987
rapC	Bacillus subtilis subsp. subtilis str. 168	57.105	99.738	0.57