Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   D9C12_RS10635 Genome accession   NZ_CP032857
Coordinates   2023773..2024666 (+) Length   297 a.a.
NCBI ID   WP_069837509.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain 2RL2-3     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2018773..2029666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C12_RS10615 (D9C12_10615) - 2019470..2021173 (+) 1704 WP_069837508.1 hypothetical protein -
  D9C12_RS10620 (D9C12_10620) ylqH 2021170..2021451 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  D9C12_RS10625 (D9C12_10625) sucC 2021624..2022781 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  D9C12_RS10630 (D9C12_10630) sucD 2022810..2023712 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  D9C12_RS10635 (D9C12_10635) dprA 2023773..2024666 (+) 894 WP_069837509.1 DNA-processing protein DprA Machinery gene
  D9C12_RS10640 (D9C12_10640) topA 2024854..2026929 (+) 2076 WP_003245599.1 type I DNA topoisomerase -
  D9C12_RS10645 (D9C12_10645) trmFO 2027005..2028312 (+) 1308 WP_069837510.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  D9C12_RS10650 (D9C12_10650) xerC 2028380..2029294 (+) 915 WP_014479777.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32972.20 Da        Isoelectric Point: 9.8131

>NTDB_id=319407 D9C12_RS10635 WP_069837509.1 2023773..2024666(+) (dprA) [Bacillus subtilis subsp. subtilis strain 2RL2-3]
MDQAAACLSICRINQLLSPSHLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQLYPKLQRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKDWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=319407 D9C12_RS10635 WP_069837509.1 2023773..2024666(+) (dprA) [Bacillus subtilis subsp. subtilis strain 2RL2-3]
TTGGATCAGGCCGCTGCCTGCCTATCGATTTGCAGAATCAATCAATTATTATCCCCATCCCATCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCCGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAACTTTATCCAAAGCTCCAGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCCGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTGGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGATTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACGAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTATTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

97.98

100

0.98
  dprA Lactococcus lactis subsp. cremoris KW2

41.697

91.246

0.38

Multiple sequence alignment